HSPA2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HSPA2 Polyklonal Antikörper für FC, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 12797-1-AP

Synonyme

Heat shock 70 kDa protein 2, heat shock 70kDa protein 2, HSP70 2, HSP70 3, HSPA2

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 12797-1-AP

  WB result of HSPA2 antibody (12797-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSPA2 transfected HeLa cells.

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  WB analysis using 12797-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12797-1-AP (HSPA2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12797-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12797-1-AP (HSPA2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12797-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12797-1-AP (HSPA2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 12797-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12797-1-AP (HSPA2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 12797-1-AP

  IP Result of anti-HSPA2 (IP:12797-1-AP, 4ug; Detection:12797-1-AP 1:1000) with mouse skeletal muscle tissue lysate 4000ug.

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  IHC staining of human testis using 12797-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 12797-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate hyperplasia using 12797-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate hyperplasia using 12797-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary tumor using 12797-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 12797-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 12797-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 12797-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HeLa using 12797-1-AP

  1X10^6 HeLa cells were stained with 0.2ug HSPA2 antibody (12797-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, C6-Zellen, HeLa-Zellen, humanes Hirngewebe, humanes Nierengewebe, humanes Skelettmuskelgewebe, Jurkat-Zellen, MCF-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Ovarialkarzinomgewebe, humanes Prostatahyperplasie-Gewebe, Maus-Eierstockgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 6 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

12797-1-AP bindet in WB, IP, IHC, IF, FC, ELISA HSPA2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HSPA2 fusion protein Ag3539
• Vollständiger Name: heat shock 70kDa protein 2
• Berechnetes Molekulargewicht: 639 aa, 70 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC036107
• Gene symbol: HSPA2
• Gene ID (NCBI): 3306
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human HSPA2 is a member of the HSPA (HSP70) family of heat-shock proteins, encoded by the gene originally described as testis-specific. Recently, it has been reported that HSPA2 can be also expressed in human somatic tissues in a cell-type specific manner. HSPA2 is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. HSPA2 is involved in sperm maturity, function and fertility. Aberrant expression of HSPA2 in testes has been connected with male infertility. Recently, HSPA2 has attracted increased interest due to its possible involvement in carcinogenesis of non-testicular tissues. This antibody well recognized the endogenous HSPA2 protein in testis and multiple cell lines. (21373891)

Publikationen

Species	Application	Title
mouse	IF	Sci Adv; Circular RNAs from BOULE play conserved roles in protection against stress-induced fertility decline.; Authors - Liuze Gao
human	IHC	Cell Death Differ; RNF144A functions as a tumor suppressor in breast cancer through ubiquitin ligase activity-dependent regulation of stability and oncogenic functions of HSPA2.; Authors - Yin-Long Yang ; KD Validated
human	WB	Curr Biol; RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.; Authors - Long Jiang
		J Mol Cell Biol; Cigarette smoke-induced cell cycle arrest in spermatocytes [GC-2spd(ts)] is mediated through cross talk between Ahr-Nrf2 pathway and MAPK signaling.; Authors - Prabagaran Esakky
mouse	WB,IHC	Development; Mutation of Eif4g3, encoding a eukaryotic translation initiation factor, causes male infertility and meiotic arrest of mouse spermatocytes.; Authors - Sun Fengyun F
human	WB, IHC	Int J Mol Sci; Various Anti-HSPA2 Antibodies Yield Different Results in Studies on Cancer-Related Functions of Heat Shock Protein A2.; Authors - Dorota Scieglinska ; KD Validated