HSPA2 Rekombinanter Antikörper

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• KD/KO Validated

HSPA2 Rekombinant Antikörper für WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240421A2

Kat-Nr. : 83466-1-PBS

Synonyme

240421A2, Heat shock 70 kDa protein 2, Heat shock protein family A member 2, Heat shock-related 70 kDa protein 2, HSP70 2

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  WB analysis using 83466-1-RR (same clone as 83466-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83466-1-RR (HSPA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83466-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 83466-1-RR (same clone as 83466-1-PBS)

  WB result of HSPA2 antibody (83466-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSPA2 transfected HeLa cells. This data was developed using the same antibody clone with 83466-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP00465-1

  Sandwich ELISA standard curve of MP00465-1, Human HSPA2 Recombinant Matched Antibody Pair - PBS only. 83466-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3539. 83466-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

• 

  Sample test of MP00465-1

  The mean HSPA2 concentration was determined to be 34.57 ng/mL in A549 cell extract based on a 2.5 mg/mL extract load.

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  IHC staining of mouse testis using 83466-1-RR (same clone as 83466-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83466-1-RR (HSPA2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83466-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 83466-1-RR (same clone as 83466-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83466-1-RR (HSPA2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83466-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00465-1

  Cytometric bead array standard curve of MP00465-1, HSPA2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83466-1-PBS. Detection antibody: 83466-3-PBS. Standard: Ag3539. Range: 0.625-80 ng/mL.

• 

  Affinity and Kinetic Characterization of 83466-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83466-1-RR against Human HSPA2 were performed. The affinity constant is 1.86 nM.

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Geprüfte Anwendungen

Produktinformation

83466-1-PBS bindet in WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test HSPA2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HSPA2 fusion protein Ag3539
• Vollständiger Name: heat shock 70kDa protein 2
• Berechnetes Molekulargewicht: 639 aa, 70 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC036107
• Gene symbol: HSPA2
• Gene ID (NCBI): 3306
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human HSPA2 is a member of the HSPA (HSP70) family of heat-shock proteins, encoded by the gene originally described as testis-specific. Recently, it has been reported that HSPA2 can be also expressed in human somatic tissues in a cell-type specific manner. HSPA2 is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. At physiological temperature, the HSPA2 was localized primarily in cytoplasm whereas, during heat shock, localization shifted to nucleus and nucleoli (PMID: 18452162). HSPA2 is involved in sperm maturity, function and fertility. Aberrant expression of HSPA2 in testes has been connected with male infertility. Recently, HSPA2 has attracted increased interest due to its possible involvement in carcinogenesis of non-testicular tissues. This antibody well recognized the endogenous HSPA2 protein in testis and multiple cell lines. (21373891)