HSPA6 Monoklonaler Antikörper

HSPA6 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

3D12D7

Kat-Nr. : 68257-1-Ig

Synonyme

Heat shock 70 kDa protein 6, Heat shock 70 kDa protein B, HSP70B, HSPA6

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  WB analysis using 68257-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68257-1-Ig (HSPA6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 68257-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 68257-1-Ig (HSPA6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 68257-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 68257-1-Ig (HSPA6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 68257-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using HSPA6 antibody (68257-1-Ig, Clone: 3D12D7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

68257-1-Ig bindet in WB, IHC, IF/ICC, ELISA HSPA6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HSPA6 fusion protein Ag4716
• Vollständiger Name: heat shock 70kDa protein 6 (HSP70B')
• Berechnetes Molekulargewicht: 643 aa, 71 kDa
• Beobachtetes Molekulargewicht: 68-71 kDa
• GenBank-Zugangsnummer: BC035665
• Gene symbol: HSPA6
• Gene ID (NCBI): 3310
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSPA6 (also known as HSP70B) is a member of the HSPA (HSP70) family of heat-shock proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Human HSP70B is known to be strictly inducible and shows very low or no basal expression levels in most cells. HSPA6 induction has been exploited as a reasonable biomarker of cellular stresses and its mRNA expression levels have been shown to be significantly induced against a variety of cellular stresses. This antibody was raised against the C-terminal region of human HSPA6 and it may cross-react with HSPA1 (HSP70), HSPA1L and HSPA2. (18229458)

Publikationen

Species	Application	Title
human	WB,IHC	Biochim Biophys Acta Mol Cell Res; PP4R1 accelerates the malignant progression of NSCLC via up-regulating HSPA6 expression and HSPA6-mediated ER stress; Authors - Xunxia Zhu