GRP75 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GRP75 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte, Zebrafisch und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(53)

Rezensionen (5)

Kat-Nr. : 14887-1-AP

Synonyme

HSPA9, 75 kDa glucose-regulated protein, CSA, GRP-75, Heat shock 70 kDa protein 9

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  WB analysis using 14887-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14887-1-AP (GRP75 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 cells using 14887-1-AP

  A549 cells (shcontrol and shRNA of GRP75)were subjected to SDS PAGE followed by western blot with 14887-1-AP (GRP75 antibody) at dilution of 1:500.

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  WB analysis of mouse testis using 14887-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14887-1-AP (GRP75 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14887-1-AP

  IP result of anti-GRP75 (IP:14887-1-AP, 4ug; Detection:14887-1-AP 1:2000) with mouse brain tissue lysate 3680ug.

• 

  IP experiment of NIH/3T3 using 14887-1-AP

  IP result of anti-GRP75 (IP:14887-1-AP, 4ug; Detection:14887-1-AP 1:700) with NIH/3T3 cells lysate 1200ug.

• 

  IHC staining of human colon using 14887-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 14887-1-AP (GRP75 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 14887-1-AP

  Immunofluorescent analysis of HepG2 cells using 14887-1-AP (GRP75 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  IF Staining of HepG2 using 14887-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GRP75 antibody (14887-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HepG2 using 14887-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.2 ug GRP75 Polyclonal antibody (14887-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, A549-Zellen, Jurkat-Zellen, Maushodengewebe, NIH/3T3-Zellen
• Erfolgreiche IP: Maushirngewebe, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: human normal colon; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 43 publications below
• IHC: See 4 publications below
• IF: See 11 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

14887-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA GRP75 und zeigt Reaktivität mit human, Maus, Ratten, Zebrafisch

• Getestete Reaktivität: human, Maus, Ratte, Zebrafisch
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GRP75 fusion protein Ag6673
• Vollständiger Name: heat shock 70kDa protein 9 (mortalin)
• Berechnetes Molekulargewicht: 74 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC000478
• Gene symbol: GRP75
• Gene ID (NCBI): 3313
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GRP75 (also known as mortalin, HSPA9 or mt-Hsp70) is a constitutively expressed member of the HSPA (HSP70) family of heat-shock proteins. It is located in the mitochondrial matrix and anti-GRP75 is commonly used as the marker for mitochondria. It has been reported that GRP75 is enriched in cancer cells and contributes to carcinogenesis. In addition, decreased expression level of GRP75 has been found in neurodegenerative disorders like Parkinson's disease. This antibody recognized the endogenous GRP75 protein in NIH/3T3 cells. (PMID: 21640711, 22920904)

Publikationen

Species	Application	Title
human	WB	Mol Cell; The mitochondrial DNAJC co-chaperone TCAIM reduces α-ketoglutarate dehydrogenase protein levels to regulate metabolism; Authors - Wang Jiahui ; KD Validated
human	WB	Cell Metab; DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling.; Authors - Ricarda Richter-Dennerlein
human	WB	Nat Commun; LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding.; Authors - Chun-Shik Shin
mouse		EMBO Mol Med; Excess hydrogen sulfide and polysulfides production underlies a schizophrenia pathophysiology.; Authors - Masayuki Ide
mouse	WB	J Cell Biol; Loss of OMA1 delays neurodegeneration by preventing stress-induced OPA1 processing in mitochondria.; Authors - Anne Korwitz
rat	WB	Cell Death Dis; Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats; Authors - Yajie Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Lenie (Verified Customer) (07-18-2025)

IF worked. WB did not on zebrafish tissue and human patient. Could you perhaps provide me with another protocol?

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1/5000 (WB), 1/200 (IF)
• Cell Tissue Type: Zebrafish

GRP75 Antibody Western Blot,Immunofluorescence validation (1/5000 (WB), 1/200 (IF) dilution) in Zebrafish (Cat no:14887-1-AP)

FH

Anastasia (Verified Customer) (08-07-2024)

Deyolked zebrafish embryos

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Zebrafish embryos

GRP75 Antibody Western Blot validation (1:1000 dilution) in Zebrafish embryos (Cat no:14887-1-AP)

FH

Christina (Verified Customer) (11-14-2023)

I used this antibody for HeLa extracts in Western Blot and worked perfectly.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HeLa cells

FH

Ning (Verified Customer) (02-27-2023)

Specifically probe endogenous GRP75 with a right MW.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse brain lysate

GRP75 Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:14887-1-AP)

FH

Staci (Verified Customer) (02-02-2023)

Antibody works very well with GRP75. Very little non-specific binding on immunoblot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: Mouse Islet protein lysates