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HSP27 Polyklonaler Antikörper
HSP27 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (3)
Anwendung
WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 18284-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | A549-Zellen, C6-Zellen, HeLa-Zellen, HepG2-Zellen, Mauslebergewebe, U2OS-Zellen |
| Erfolgreiche Detektion in IHC | humanes Lungenkarzinomgewebe, humanes Gliomgewebe, humanes Leberkarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF/ICC | HeLa-Zellen, MCF-7-Zellen |
| Erfolgreiche Detektion in FC (Intra) | MCF-7-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunhistochemie (IHC) | IHC : 1:50-1:500 |
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 |
| Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 4 publications below |
| WB | See 33 publications below |
| IHC | See 6 publications below |
| IF | See 5 publications below |
| IP | See 1 publications below |
| ELISA | See 1 publications below |
| CoIP | See 1 publications below |
Produktinformation
18284-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA HSP27 und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Fisch, Hausschwein, Huhn, Maus, Ratte |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | HSP27 fusion protein Ag13161 |
| Vollständiger Name | heat shock 27kDa protein 1 |
| Berechnetes Molekulargewicht | 19-23 kDa |
| Beobachtetes Molekulargewicht | 27 kDa |
| GenBank-Zugangsnummer | BC012768 |
| Gene symbol | HSPB1 |
| Gene ID (NCBI) | 3315 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
HSPB1, also known as heat shock protein 27 (HSP27), belongs to the small heat shock protein family which is induced in response to environmental challenges or/and developmental transitions. It is also an anti-apoptotic protein that plays crucial roles in tumorigenesis and cell survival and is reported to be an independent prognosis marker for cancer. Recently HSPB1 has been found to be a valuable marker for melanoma. In addition to the predicted 27 kDa, an extra 50-55 kDa representing dimeric form of HSPB1 may also be observed (PMID: 21353161). It's notable that mouse HSP27 is also named HSP25 and has the predicted MW between 19-23 kDa.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for HSP27 antibody 18284-1-AP | Protokoll herunterladen |
| IHC protocol for HSP27 antibody 18284-1-AP | Protokoll herunterladenl |
| IF protocol for HSP27 antibody 18284-1-AP | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Allergy ACE2 downregulation in olfactory mucosa: Eosinophilic rhinosinusitis as COVID-19 protective factor? | ||
Autophagy ATG4B/autophagin-1 regulates intestinal homeostasis and protects mice from experimental colitis. | ||
J Cell Biol Human myocytes are protected from titin aggregation-induced stiffening by small heat shock proteins. | ||
Cell Rep Analysis of proteome-wide degradation dynamics in ALS SOD1 iPSC-derived patient neurons reveals disrupted VCP homeostasis | ||
Phytomedicine Ginsenoside Rg5 inhibits glioblastoma by activating ferroptosis via NR3C1/HSPB1/NCOA4 |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Tom (Verified Customer) (09-28-2020) | HEK293T lysate (10ug) ran on 4-20% gradient gel. Blocking in 5% BSA for 1 hour, followed by HSP27 (1:1000 in block) overnight at 4 degrees. Goat anti-rabbit HRP (1:10,000) for 1 hour in block.
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