Phospho-HSP27 (Ser15) Rekombinanter Antikörper

Phospho-HSP27 (Ser15) Rekombinant Antikörper für WB, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2D4

Kat-Nr. : 83332-3-PBS

Synonyme

HSP27, HSPB1, Hsp25, HMN2B, Estrogen-regulated 24 kDa protein

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  WB analysis using 83332-3-RR (same clone as 83332-3-PBS)

  Non-treated HeLa cells, and anisomycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 83332-3-RR (Phospho-HSP27 (Ser15) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin(66009-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 83332-3-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83332-3-RR (same clone as 83332-3-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Anisomycin treated HeLa cells using Phospho-HSP27 (Ser15) antibody (83332-3-RR, Clone: 2D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83332-3-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83332-3-RR (same clone as 83332-3-PBS)

  1X10^6 HeLa cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Phospho-HSP27 (Ser15) Recombinant antibody (83332-3-RR, Clone:2D4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 83332-3-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

83332-3-PBS bindet in WB, IF/ICC, FC (Intra), Indirect ELISA Phospho-HSP27 (Ser15) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: heat shock 27kDa protein 1
• Berechnetes Molekulargewicht: 19-23 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC012768
• Gene symbol: HSPB1
• Gene ID (NCBI): 3315
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSPB1, also named as HSP27, belongs to the mammalian small HSP family widely expressed in cells including the cytoplasm and nucleus. It has been reported that HSPB1 plays a key role in stress resistance, actin organization and numerous biological processes. HSPB1 can be phosphorylated in different stimulation such as cytokines, growth factors and peptide hormones on the site of Ser15, Ser78 and Ser82 by MAPKAP2 kinase. The phosphorylation of HSPB1 leads to a decrease in the size of HSPB1 oligomers. HSPB1 is associated with amyotrophic lateral sclerosis (ALS) (PMID: 8542580, 11960785, 10383393, 32231288)