HSPB11 Polyklonaler Antikörper

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• KD/KO Validated

HSPB11 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

17

Kat-Nr. : 15732-1-AP

Synonyme

C1orf41, Heat shock protein beta 11, HSPB11, HSPCO34, IFT25, ITF25, Placental protein 25, PP25

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Galerie der Validierungsdaten

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  WB analysis using 15732-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15732-1-AP (HSPB11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Produktinformation

15732-1-AP bindet in WB, IHC, IF, ELISA HSPB11 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HSPB11 fusion protein Ag8490
• Vollständiger Name: heat shock protein family B (small), member 11
• Berechnetes Molekulargewicht: 144 aa, 16 kDa
• Beobachtetes Molekulargewicht: 16-20 kDa
• GenBank-Zugangsnummer: BC005245
• Gene symbol: HSPB11
• Gene ID (NCBI): 51668
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSPB11 (Heat shock protein beta-11), also known as IFT25, was originally identified as a small heat shock protein. Recently it has been identified as a component of IFT complex B. It has been demonstrated that IFT25 is not required for ciliary assembly but is required for proper Hedgehog signaling, which in mammals occurs within cilia. Cilia lacking IFT25 have defects in the signal-dependent transport of multiple Hedgehog components and fail to activate the pathway upon stimulation.

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis.; Authors - Malavika Raman ; KD Validated
mouse	WB,IF	Dev Cell; IFT25 links the signal-dependent movement of Hedgehog components to intraflagellar transport.; Authors - Keady Brian T BT
mouse	WB,IF	Dev Cell; IFT27 Links the BBSome to IFT for Maintenance of the Ciliary Signaling Compartment.; Authors - Thibaut Eguether
mouse	WB	EMBO J; Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.; Authors - Shichao Duan
mouse	WB	Curr Biol; Rab34 is necessary for early stages of intracellular ciliogenesis.; Authors - Michael W Stuck
human	WB	Dev Cell; The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.; Authors - Tomoharu Kanie

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