HSPB11 Monoklonaler Antikörper

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• KD/KO Validated

HSPB11 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

2D11B3

Kat-Nr. : 68059-1-Ig

Synonyme

Heat shock protein beta-11, Heat shock protein beta 11, FAP232, CFAP232, C1orf41

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  WB analysis using 68059-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68059-1-Ig (HSPB11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 68059-1-Ig

  WB result of HSPB11 antibody (68059-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSPB11 transfected HeLa cells.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NCCIT-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, T-47D-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68059-1-Ig bindet in WB, ELISA HSPB11 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HSPB11 fusion protein Ag8804
• Vollständiger Name: heat shock protein family B (small), member 11
• Berechnetes Molekulargewicht: 144 aa, 16 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC005245
• Gene symbol: HSPB11
• Gene ID (NCBI): 51668
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSPB11 (Heat shock protein beta-11), also known as IFT25, was originally identified as a small heat shock protein. Recently it has been identified as a component of IFT complex B. It has been demonstrated that IFT25 is not required for ciliary assembly but is required for proper Hedgehog signaling, which in mammals occurs within cilia. Cilia lacking IFT25 have defects in the signal-dependent transport of multiple Hedgehog components and fail to activate the pathway upon stimulation.