HSPB8 Polyklonaler Antikörper

HSPB8 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(14)

Kat-Nr. : 15287-1-AP

Synonyme

E2-induced gene 1 protein, E2IG1, DHMN2, CRYAC, CMT2L

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  WB analysis using 15287-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15287-1-AP (HSPB8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 15287-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 15287-1-AP (HSPB8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15287-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15287-1-AP (HSPB8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15287-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15287-1-AP (HSPB8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 15287-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Neuro-2a-Zellen, HEK-293-Zellen, Maushirngewebe, Mausherzgewebe, Rattenherzgewebe, SGC-7901-Zellen
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Hirngewebe, humanes Nierengewebe, humanes Lungengewebe, humanes Eierstockgewebe, humanes Plazenta-Gewebe, humanes Hautgewebe, humanes Milzgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

15287-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA HSPB8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HSPB8 fusion protein Ag7456
• Vollständiger Name: heat shock 22kDa protein 8
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 20-22 kDa
• GenBank-Zugangsnummer: BC002673
• Gene symbol: HSPB8
• Gene ID (NCBI): 26353
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Heat Shock Protein B8 (HSPB8), also named small stress protein-like protein (sHSP22), protein kinase H11(H11), E2-induced gene 1 protein (E2IG1), or alpha-crystallin C chain (CRYAC), is a small chaperone involved in chaperone-assisted selective autophagy (CASA) (PMID: 33562660). HSPB8 is expressed at relatively high levels in the heart and the skeletal muscle tissue. HSPB8 is composed of 196 amino acids with an apparent molecular mass of 21.6 kDa (PMID: 20570967).

Publikationen

Species	Application	Title
human	WB, IF	Nat Commun; Cardiomyocyte contractile impairment in heart failure results from reduced BAG3-mediated sarcomeric protein turnover.; Authors - Thomas G Martin
mouse	IHC	J Neurosci; The α crystallin domain of small heat shock protein b8 (Hspb8) acts as survival and differentiation factor in adult hippocampal neurogenesis.; Authors - Ramírez-Rodríguez Gerardo G
human	WB	J Am Heart Assoc; Reduction of Filamin C Results in Altered Proteostasis, Cardiomyopathy, and Arrhythmias; Authors - Joyce C Ohiri
mouse	WB	J Cell Biochem; Pharmacological inhibition of BAG3-HSP70 with the proposed cancer therapeutic JG-98 is toxic for cardiomyocytes.; Authors - Thomas G Martin
mouse,human	CoIP	Genes (Basel); BAG3 Alleviates Atherosclerosis by Inhibiting Endothelial-to-Mesenchymal Transition via Autophagy Activation.; Authors - Hongtao Diao
human	WB	Cell Rep; Stress-induced perturbations in intracellular amino acids reprogram mRNA translation in osmoadaptation independently of the ISR.; Authors - Dawid Krokowski