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HSP60 Rekombinanter Antikörper

HSP60 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250248D1

Kat-Nr. : 86275-1-PBS

Synonyme

HSPD1, 60 kDa chaperonin, Chaperonin 60, CPN60, EC:5.6.1.7

Filter:
  • Western Blot (WB) analysis of various lysates using HSP60 Recombinant monoclonal antibody (86275-1-RR)

    WB analysis using 86275-1-RR (same clone as 86275-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86275-1-RR (HSP60 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86275-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HSP60 Recombinant monoclonal antibody (86275-1-RR)

    IF Staining of HeLa using 86275-1-RR (same clone as 86275-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using HSP60 antibody (86275-1-RR, Clone: 250248D1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86275-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86275-1-RR

    Affinity and Kinetic Characterization of 86275-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86275-1-RR against Human HSPD1 were performed. The affinity constant is 7.43 nM.

Geprüfte Anwendungen

Produktinformation

86275-1-PBS bindet in WB, IF/ICC, Indirect ELISA HSP60 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen HSP60 fusion protein Ag7401
Vollständiger Name heat shock 60kDa protein 1 (chaperonin)
Berechnetes Molekulargewicht 61 kDa
Beobachtetes Molekulargewicht 60 kDa
GenBank-ZugangsnummerBC003030
Gene symbol HSP60
Gene ID (NCBI) 3329
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSPD1, also known as HSP60, belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415)