HSPH1 Polyklonaler Antikörper

HSPH1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 13383-1-AP

Synonyme

Antigen NY CO 25, Heat shock 110 kDa protein, Heat shock protein 105 kDa, HSP105, HSP105A, HSP105B, HSP110, HSPH1, KIAA0201, NY CO 25

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 13383-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13383-1-AP (HSPH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13383-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13383-1-AP (HSPH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13383-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13383-1-AP (HSPH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 13383-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13383-1-AP (HSPH1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13383-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13383-1-AP (HSPH1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 13383-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 13383-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 13383-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 13383-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 13383-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 13383-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 13383-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 13383-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 13383-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 13383-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF7 cells, HeLa-Zellen, humanes Hirngewebe, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 3 publications below

Produktinformation

13383-1-AP bindet in WB, IHC, IF, ELISA HSPH1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HSPH1 fusion protein Ag4224
• Vollständiger Name: heat shock 105kDa/110kDa protein 1
• Berechnetes Molekulargewicht: 858 aa, 97 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC037553
• Gene symbol: HSPH1
• Gene ID (NCBI): 10808
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSP105, also known as HSP110 or HSPH1, belongs to the heat shock protein (HSP) family. Human HSP105 is a high-molecular-weight chaperone protein expressed at constitutively low levels as a cytoplasmic α-isoform and as an inducible nuclear β-isoform on exposure to various forms of stress. HSP105 is constitutively overexpressed in several solid tumors, including melanoma, breast, thyroid, and gastroenteric cancers, and exerts antiapoptotic functions. Recently HSP105 has been identified as a novel candidate biomarker of lymphoma aggressiveness. This antibody recognizes both HSP105α and HSP105β isoforms. Western blot analysis using this antibody detected a major band around 100-110 kDa in Jurkat cells.

Publikationen

Species	Application	Title
human	IF	Nat Cell Biol; Heat-shock chaperone HSPB1 regulates cytoplasmic TDP-43 phase separation and liquid-to-gel transition; Authors - Shan Lu
mouse	WB	Mol Ther Nucleic Acids; Age-associated changes in microglia and astrocytes ameliorate blood-brain barrier dysfunction; Authors - Jie Pan
mouse	WB	Mol Reprod Dev; Identification of Hsf1 as a novel androgen receptor-regulated gene in mouse sertoli cells.; Authors - Lihua Yang
human	IF	bioRxiv; Endothelial Cells are Heterogeneous in Different Brain Regions and are Dramatically Altered in Alzheimer's Disease; Authors - Annie Bryant
human	IF	J Neurosci; Endothelial Cells Are Heterogeneous in Different Brain Regions and Are Dramatically Altered in Alzheimer's Disease; Authors - Annie Bryant
human,mouse		Dev Cell; Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination; Authors - Li Li