HSPH1 Polyklonaler Antikörper

HSPH1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13383-1-PBS

Synonyme

KIAA0201, HSP110, HSP105, Heat shock protein family H member 1, Antigen NY-CO-25

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  WB analysis of MCF-7 using 13383-1-AP (same clone as 13383-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13383-1-AP (HSPH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13383-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 13383-1-AP (same clone as 13383-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13383-1-AP (HSPH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13383-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 13383-1-AP (same clone as 13383-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 13383-1-AP (HSPH1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13383-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 13383-1-AP (same clone as 13383-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 13383-1-AP (HSPH1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13383-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 13383-1-AP (same clone as 13383-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13383-1-AP (HSPH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13383-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 13383-1-AP (same clone as 13383-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13383-1-AP (HSPH1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13383-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 13383-1-AP (same clone as 13383-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13383-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 13383-1-AP (same clone as 13383-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13383-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 13383-1-AP (same clone as 13383-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13383-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 13383-1-AP (same clone as 13383-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13383-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 13383-1-AP (same clone as 13383-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13383-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 13383-1-AP (same clone as 13383-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13383-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 13383-1-AP (same clone as 13383-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 13383-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 13383-1-AP (same clone as 13383-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 13383-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 13383-1-AP (same clone as 13383-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 13383-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 13383-1-AP (same clone as 13383-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 13383-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13383-1-PBS bindet in WB, IHC, Indirect ELISA HSPH1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HSPH1 fusion protein Ag4224
• Vollständiger Name: heat shock 105kDa/110kDa protein 1
• Berechnetes Molekulargewicht: 858 aa, 97 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC037553
• Gene symbol: HSPH1
• Gene ID (NCBI): 10808
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSP105, also known as HSP110 or HSPH1, belongs to the heat shock protein (HSP) family. Human HSP105 is a high-molecular-weight chaperone protein expressed at constitutively low levels as a cytoplasmic α-isoform and as an inducible nuclear β-isoform on exposure to various forms of stress. HSP105 is constitutively overexpressed in several solid tumors, including melanoma, breast, thyroid, and gastroenteric cancers, and exerts antiapoptotic functions. Recently HSP105 has been identified as a novel candidate biomarker of lymphoma aggressiveness. This antibody recognizes both HSP105α and HSP105β isoforms. Western blot analysis using this antibody detected a major band around 100-110 kDa in Jurkat cells.