HTATSF1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HTATSF1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 20805-1-AP

Synonyme

HIV 1 Tat specific factor 1, HIV Tat specific factor 1, HTATSF1, TAT SF1

---

Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 20805-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20805-1-AP (HTATSF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 20805-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 20805-1-AP (HTATSF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20805-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20805-1-AP (HTATSF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20805-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20805-1-AP (HTATSF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 20805-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20805-1-AP (HTATSF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20805-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20805-1-AP (HTATSF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20805-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20805-1-AP (HTATSF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 20805-1-AP

  IP Result of anti-HTATSF1 (IP:20805-1-AP, 4ug; Detection:20805-1-AP 1:2000) with HepG2 cells lysate 2400ug.

• 

  IHC staining of human breast cancer using 20805-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20805-1-AP (HTATSF1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 20805-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20805-1-AP (HTATSF1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 20805-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20805-1-AP (HTATSF1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 20805-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20805-1-AP (HTATSF1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 20805-1-AP

  Immunofluorescent analysis of Hela cells, using HTATSF1 antibody 20805-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HeLa using 20805-1-AP

  Immunofluorescent analysis of Hela cells, using HTATSF1 antibody 20805-1-AP at 1:50 dilution and FITC- labeled FITC-Donkey anti-Rabbit IgG (green).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Produktinformation

20805-1-AP bindet in WB, IP, IHC, IF, ELISA HTATSF1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HTATSF1 fusion protein Ag14744
• Vollständiger Name: HIV-1 Tat specific factor 1
• Berechnetes Molekulargewicht: 755 aa, 86 kDa
• Beobachtetes Molekulargewicht: 130-140 kDa
• GenBank-Zugangsnummer: BC009896
• Gene symbol: HTATSF1
• Gene ID (NCBI): 27336
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Mol Cell; A PARylation-phosphorylation cascade promotes TOPBP1 loading and RPA-RAD51 exchange in homologous recombination.; Authors - Jiao Zhao ; KD Validated
		Nucleic Acids Res; Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II.; Authors - Xiaodong Lu
human	WB,IHC,IP	Cell Death Discov; Metabolic protein phosphoglycerate kinase 1 confers lung cancer migration by directly binding HIV Tat specific factor 1.; Authors - Yu-Chan Chang ; KD Validated