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HTR3A Polyklonaler Antikörper

HTR3A Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29685-1-AP

Synonyme

5 HT 3, 5 HT3 A, 5 HT3A, 5 HT3R, 5 hydroxytryptamine receptor 3

Filter:
  • Western Blot (WB) analysis of various lysates using HTR3A Polyclonal antibody (29685-1-AP)

    WB analysis using 29685-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29685-1-AP (HTR3A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBMauslungengewebe, Maushodengewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

29685-1-AP bindet in WB, ELISA HTR3A und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen HTR3A fusion protein Ag31317
Vollständiger Name 5-hydroxytryptamine (serotonin) receptor 3A
Berechnetes Molekulargewicht 55 kDa
Beobachtetes Molekulargewicht55 kDa
GenBank-ZugangsnummerBC002354
Gene symbol HTR3A
Gene ID (NCBI) 3359
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HTR3A (5-hydroxytryptamine receptor 3A, 5-HT3A) belongs to the ligand-gated ion channel receptor superfamily. It is the subunit A of the type 3 receptor for 5-hydroxytryptamine (5-HT, serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. So far, five distinct 5-HT3 receptor subunits (A-E) have been identified. HTR3A can form functional homomers or heteromers with HTR3B or HTR3C or HTR3D or HTR3E. 5-HT3 receptors are capable of mediating fast excitatory neurotransmission in the CNS and peripheral nervous system (PNS). (PMID: 23038271; 17073663)

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for HTR3A antibody 29685-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Anna (Verified Customer) (10-04-2022)

Large background, no specific reaction. I have a problem with attaching a photo file. https://drive.google.com/file/d/15d3t6thx7rcWyN-_DkkKCtpAMyi8jxHO/view

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 50, 100, 250, 500
  • Cell Tissue Type: brain frozen sections - mouse