HTR7 Polyklonaler Antikörper

HTR7 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Rezensionen (1)

Kat-Nr. : 13830-1-AP

Synonyme

5 HT 7, 5 HT X, 5 HT7, 5 hydroxytryptamine receptor 7, HTR7, Serotonin receptor 7

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  WB analysis of mouse brain using 13830-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13830-1-AP (HTR7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 13830-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13830-1-AP (HTR7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 13830-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13830-1-AP (HTR7 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 13830-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13830-1-AP (HTR7 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Mausmilzgewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• FC: See 1 publications below

Produktinformation

13830-1-AP bindet in WB, IHC, IF, ELISA HTR7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HTR7 fusion protein Ag4742
• Vollständiger Name: 5-hydroxytryptamine (serotonin) receptor 7 (adenylate cyclase-coupled)
• Berechnetes Molekulargewicht: 445 aa, 49 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC047526
• Gene symbol: HTR7
• Gene ID (NCBI): 3363
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Serotonin (5-hydroxytryptamine; 5-HT) acts as a neurotransmitter and neuromodulator in the central nervous system(CNS) (PMID:23100172). The behavioral effects of 5-HT are mediated by a large family of specific 5-HT receptors. HTR7 (5-hydroxytryptamine receptor 7) is a G protein-coupled receptor (GPCR), and many transcription factors can bind to its promoter, such as AP2, EGR-1, and MAZ (PMID:34164474). It is located in different brain regions, such as the hypothalamus, thalamus, cerebral cortex, and hippocampus (PMID:29637287). HTR7 is associated with circadian rhythm, anxiety, depression, schizophrenia, nociception, and memory (PMID:9154233).

Publikationen

Species	Application	Title
human	IHC	Cell Rep Med; Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application; Authors - Xiaoxue Ren
mouse	WB	Mol Psychiatry; ZFP804A mutant mice display sex-dependent schizophrenia-like behaviors.; Authors - Ying Huang
human	FC	Biomedicines; T Lymphocyte Serotonin 5-HT7 Receptor Is Dysregulated in Natalizumab-Treated Multiple Sclerosis Patients; Authors - Flora Reverchon
human	WB	Onco Targets Ther; 5-HT7 Receptor Contributes to Proliferation, Migration and Invasion in NSCLC Cells.; Authors - Xiaohui Du
mouse	WB	Biochem Biophys Res Commun; FSH signaling is involved in affective disorders.; Authors - Wen-Kai Bi
mouse	IHC,IF	Cancer Lett; Serotonylation in tumor-associated fibroblasts contributes to the tumor-promoting roles of serotonin in colorectal cancer; Authors - Tianlong Ling

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Clarissa (Verified Customer) (05-08-2019)

Good antibody, stained with goat  anti-Rabbit IgG (H+L) Secondary Antibody, Texas Red-X.

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: rabbit spinal cord

HTR7 Antibody Immunohistochemistry,Immunofluorescence, validation (1:1000 dilution) in rabbit spinal cord (Cat no:13830-1-AP)