HTRA2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HTRA2 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15775-1-PBS

Synonyme

---

• 

  WB analysis of HeLa using 15775-1-AP (same clone as 15775-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15775-1-AP (HTRA2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 15775-1-AP (same clone as 15775-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 15775-1-AP (HTRA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 15775-1-AP (same clone as 15775-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15775-1-AP (HTRA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 15775-1-AP (same clone as 15775-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 15775-1-AP (HTRA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

• 

  IP experiment of Raji using 15775-1-AP (same clone as 15775-1-PBS)

  IP result of anti-HTRA2 (IP:15775-1-AP, 4ug; Detection:15775-1-AP 1:500) with Raji cells lysate 2000ug. This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 15775-1-AP (same clone as 15775-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 15775-1-AP (HTRA2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 15775-1-AP (same clone as 15775-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 15775-1-AP (HTRA2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 15775-1-AP (same clone as 15775-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 15775-1-AP (same clone as 15775-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid using 15775-1-AP (same clone as 15775-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid using 15775-1-AP (same clone as 15775-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 15775-1-AP (same clone as 15775-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 15775-1-AP (same clone as 15775-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15775-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

15775-1-PBS bindet in WB, IHC, IP, Indirect ELISA HTRA2 und zeigt Reaktivität mit Affe, human, Maus, Ratten

• Getestete Reaktivität: Affe, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HTRA2 fusion protein Ag8455
• Vollständiger Name: HtrA serine peptidase 2
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC000096
• Gene symbol: HTRA2
• Gene ID (NCBI): 27429
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HTRA2(High temperature requirement protein A2) is also named as OMI, PRSS25 and belongs to the peptidase S1B family. HTRA2 normally exists in the mitochondria, can cause MMP3 activation in the cytosol under a cell stress condition, which can ultimately lead to demise of dopaminergic neuronal cells(PMID: 22265821). It inhibits mitochondrial superoxide generation, stabilizes mitochondrial membrane potential, and prevents apoptosis at baseline and in response to extracellular inducers of mitochondrial stress(PMID: 18772386). This protein has 4 isoforms produced by alternative splicing.