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HTRA2 Rekombinanter Antikörper

HTRA2 Rekombinant Antikörper für IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240496E12

Kat-Nr. : 83492-6-PBS

Synonyme

PARK13, Omi stress-regulated endoprotease, OMI, HtrA serine peptidase 2, EC:3.4.21.108

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HTRA2 Recombinant antibody (83492-6-RR)

    IF Staining of HepG2 using 83492-6-RR (same clone as 83492-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HTRA2 antibody (83492-6-RR, Clone: 240496E12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83492-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using HTRA2 Recombinant antibody (83492-6-RR)

    FC experiment of HeLa using 83492-6-RR (same clone as 83492-6-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HTRA2 Recombinant antibody (83492-6-RR, Clone:240496E12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83492-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83492-6-RR

    Affinity and Kinetic Characterization of 83492-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83492-6-RR against Human HTRA2 were performed. The affinity constant is 9.83 nM.

Geprüfte Anwendungen

Produktinformation

83492-6-PBS bindet in IF/ICC, FC (Intra), ELISA HTRA2 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen HTRA2 fusion protein Ag8455
Vollständiger Name HtrA serine peptidase 2
Berechnetes Molekulargewicht 49 kDa
GenBank-ZugangsnummerBC000096
Gene symbol HTRA2
Gene ID (NCBI) 27429
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.