HVCN1 Monoklonaler Antikörper

HVCN1 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF

Konjugation

Unkonjugiert

CloneNo.

1E4C4

Kat-Nr. : 66449-1-Ig

Synonyme

HV1, HVCN1, VSOP

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Galerie der Validierungsdaten

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  WB analysis of Raji using 66449-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66449-1-Ig (HVCN1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66449-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66449-1-Ig (HVCN1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 66449-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66449-1-Ig (HVCN1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66449-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66449-1-Ig (HVCN1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 66449-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using HVCN1 antibody (66449-1-Ig, Clone: 1E4C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human tonsillitis using 66449-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using HVCN1 antibody (66449-1-Ig, Clone: 1E4C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66449-1-Ig bindet in WB, IHC, IF HVCN1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HVCN1 fusion protein Ag5350
• Vollständiger Name: hydrogen voltage-gated channel 1
• Berechnetes Molekulargewicht: 273 aa, 32 kDa
• Beobachtetes Molekulargewicht: 28-35 kDa, 40 kDa, 60 kDa
• GenBank-Zugangsnummer: BC032672
• Gene symbol: HVCN1
• Gene ID (NCBI): 84329
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HVCN1, also named as VSOP and HV1, Belongs to the hydrogen channel family. HVCN1 mediates the voltage-dependent proton permeability of excitable membranes. It forms a proton-selective channel through which protons may pass in accordance with their electrochemical gradient. Proton efflux, HVCN1 is accompanied by membrane depolarization, facilitates acute production of reactive oxygen species in phagocytosis. HVCN1, the voltage-sensitive proton channel, is present in human sperm and is an important regulator of the functional maturation of sperm. HVCN1 has four isoforms with MW 28-32 kDa or 40 kDa (modification). It has a dimer form with MW 60 kDa.