HVEM/TNFRSF14 Rekombinanter Antikörper

HVEM/TNFRSF14 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250728B12

Kat-Nr. : 81078-6-RR

Synonyme

TNFRSF14, ATAR, CD270, Herpes virus entry mediator A, Herpesvirus entry mediator A

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  WB analysis of human urine using 81078-6-RR

  human urine sample was subjected to SDS PAGE followed by western blot with 81078-6-RR (HVEM/TNFRSF14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 81078-6-RR

  Biolayer interferometry (BLl) kinetic assays of 81078-6-RR against Human HVEM/TNFRSF14 were performed. The affinity constant is 3.00 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human urine

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

81078-6-RR bindet in WB, ELISA HVEM/TNFRSF14 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HVEM/TNFRSF14 fusion protein Eg1275
• Vollständiger Name: tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator)
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC002794
• Gene symbol: TNFRSF14
• Gene ID (NCBI): 8764
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The mammalian tumor necrosis factor receptor (TNFR) family consists of 10 cell-surface proteins that regulate development and homeostasis of the immune system. Member 14 of TNFR (TNFRSF14, also named as TR2, ATAR, HVEA, HVEM, LIGHTR) was also identified as a mediator of herpesvirus entry into mammalian cells. The cytoplasmic region of TNFRSF14 bound to several members of the TNFR-associated factor (TRAF) family, namely TRAF1, TRAF2, TRAF3, and TRAF5. Transient transfection into human 293 cells caused marked activation of nuclear factor- B (NF- B), a transcriptional regulator of multiple immunomodulatory and inflammatory genes.