HVEM/TNFRSF14 Rekombinanter Antikörper

HVEM/TNFRSF14 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

250728C11

Kat-Nr. : 98362-2-RR

Synonyme

TNFRSF14, ATAR, CD270, Herpes virus entry mediator A, Herpesvirus entry mediator A

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  FC experiment of human peripheral blood leukocytes using 98362-2-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human HVEM/TNFRSF14 Rabbit RecAb (98362-2-RR, Clone: 250728C11) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using 98362-2-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human HVEM/TNFRSF14 Rabbit RecAb (98362-2-RR, Clone: 250728C11) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  Affinity and Kinetic Characterization of 98362-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98362-2-RR against Human HVEM/TNFRSF14 were performed. The affinity constant is 2.22 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: human peripheral blood leukocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98362-2-RR bindet in FC HVEM/TNFRSF14 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HVEM/TNFRSF14 fusion protein Eg1275
• Vollständiger Name: tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator)
• Berechnetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC002794
• Gene symbol: TNFRSF14
• Gene ID (NCBI): 8764
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

The mammalian tumor necrosis factor receptor (TNFR) family consists of 10 cell-surface proteins that regulate development and homeostasis of the immune system. Member 14 of TNFR (TNFRSF14, also named as TR2, ATAR, HVEA, HVEM, LIGHTR) was also identified as a mediator of herpesvirus entry into mammalian cells. The cytoplasmic region of TNFRSF14 bound to several members of the TNFR-associated factor (TRAF) family, namely TRAF1, TRAF2, TRAF3, and TRAF5. Transient transfection into human 293 cells caused marked activation of nuclear factor- B (NF- B), a transcriptional regulator of multiple immunomodulatory and inflammatory genes.