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  • KD/KO Validated

HYAL2 Polyklonaler Antikörper

HYAL2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 15115-1-AP

Synonyme

hyaluronoglucosaminidase 2, Hyaluronidase-2, Hyaluronidase 2, Hyal-2, Hyal 2



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMausherzgewebe, HeLa-Zellen, Mauslebergewebe, Raji-Zellen
Erfolgreiche Detektion in IHChumanes Lungenkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

15115-1-AP bindet in WB, IHC, IF/ICC, ELISA HYAL2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen HYAL2 fusion protein Ag7157
Vollständiger Name hyaluronoglucosaminidase 2
Berechnetes Molekulargewicht 54 kDa
Beobachtetes Molekulargewicht 54-60 kDa
GenBank-ZugangsnummerBC000692
Gene symbol HYAL2
Gene ID (NCBI) 8692
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hyaluronic acid (HA) is a glycosaminoglycan that is believed to have numerous important biologic functions, including modulation of cell proliferation, migration, and differentiation, as well as the regulation of extracellular water and protein homeostasis. It is also an integral structural component of cartilage and other tissues and acts as a lubricant in joints. Hyaluronidases are a family of enzymes that catalyse the degradation of HA. In humans, there are five functional hyaluronidases: HYAL1, HYAL2, HYAL3, HYAL4 and HYAL5 (also known as SPAM1 or PH-20); plus a pseudogene, HYAL6 (also known as HYALP1). HYAL2 is present in many tissues except the adult brain (PMID: 11731268). HYAL2 can be exposed to cell surfaces that bind to the plasma membrane via GPI anchors (PMID: 11731268).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for HYAL2 antibody 15115-1-APProtokoll herunterladen
IHC protocol for HYAL2 antibody 15115-1-APProtokoll herunterladenl
IF protocol for HYAL2 antibody 15115-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB,IF

Genet Med

Elucidating the clinical spectrum and molecular basis of HYAL2 deficiency.

Authors - James Fasham
  • KO Validated
humanIHC,IF,WB

Front Immunol

Complement protein C1q stimulates hyaluronic acid degradation via gC1qR/HABP1/p32 in malignant pleural mesothelioma

Authors - Andrea Balduit
MOUSEWB

PLoS Genet

PMID: 28081210

Authors - Martina M A Muggenthaler
  • KO Validated
humanIHC

Cancers (Basel)

Impact of Hyaluronic Acid on the Cutaneous T-Cell Lymphoma Microenvironment: A Novel Anti-Tumor Mechanism of Bexarotene

Authors - Tetsuya Ikawa
mouseWB

Dermatol Ther

Role of Momordica charantia in preventing the natural aging process of skin and sexual organs in mice.

Authors - Keiichi Hiramoto
mouseWB,IHC

Matrix Biol

Hyaluronan degradation by HYAL2 is essential for odontoblastic differentiation and migration of mouse dental papilla cells

Authors - Haiyan Huang