HYAL2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HYAL2 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 15115-1-AP

Synonyme

Hyal 2, HYAL2, Hyaluronidase 2, hyaluronoglucosaminidase 2, LuCa 2, LUCA2, Lung carcinoma protein 2

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Galerie der Validierungsdaten

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  WB analysis of Raji using 15115-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15115-1-AP (HYAL2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 15115-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15115-1-AP (HYAL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15115-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15115-1-AP (HYAL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 15115-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15115-1-AP (HYAL2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 15115-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15115-1-AP (HYAL2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15115-1-AP

  Immunofluorescent analysis of Hela cells, using HYAL2 antibody 15115-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HeLa using 15115-1-AP

  1X10^6 HeLa cells were stained with 0.2ug HYAL2 antibody (15115-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, HeLa-Zellen, Mauslebergewebe, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

15115-1-AP bindet in WB, IHC, IF, FC, ELISA HYAL2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HYAL2 fusion protein Ag7157
• Vollständiger Name: hyaluronoglucosaminidase 2
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 54-60 kDa
• GenBank-Zugangsnummer: BC000692
• Gene symbol: HYAL2
• Gene ID (NCBI): 8692
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hyaluronic acid (HA) is a glycosaminoglycan that is believed to have numerous important biologic functions, including modulation of cell proliferation, migration, and differentiation, as well as the regulation of extracellular water and protein homeostasis. It is also an integral structural component of cartilage and other tissues and acts as a lubricant in joints. Hyaluronidases are a family of enzymes that catalyse the degradation of HA. In humans, there are five functional hyaluronidases: HYAL1, HYAL2, HYAL3, HYAL4 and HYAL5 (also known as SPAM1 or PH-20); plus a pseudogene, HYAL6 (also known as HYALP1). HYAL2 is present in many tissues except the adult brain (PMID: 11731268). HYAL2 can be exposed to cell surfaces that bind to the plasma membrane via GPI anchors (PMID: 11731268).

Publikationen

Species	Application	Title
mouse	WB,IF	Genet Med; Elucidating the clinical spectrum and molecular basis of HYAL2 deficiency.; Authors - James Fasham ; KO Validated
human	IHC,IF,WB	Front Immunol; Complement protein C1q stimulates hyaluronic acid degradation via gC1qR/HABP1/p32 in malignant pleural mesothelioma; Authors - Andrea Balduit
MOUSE	WB	PLoS Genet; PMID: 28081210; Authors - Martina M A Muggenthaler ; KO Validated
mouse	WB	Dermatol Ther; Role of Momordica charantia in preventing the natural aging process of skin and sexual organs in mice.; Authors - Keiichi Hiramoto

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