HYAL2 Polyklonaler Antikörper

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• KD/KO Validated

HYAL2 Polyklonal Antikörper für WB, IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 51148-1-AP

Synonyme

Hyal 2, HYAL2, Hyaluronidase 2, hyaluronoglucosaminidase 2, LuCa 2, LUCA2, Lung carcinoma protein 2

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  WB analysis of mouse heart using 51148-1-AP

  mouse heart tissue and mouse liver tissue were subjected to SDS PAGE followed by western blot with 51148-1-AP (HYAL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 51148-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 51148-1-AP (HYAL2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human lung using 51148-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 51148-1-AP (HYAL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 51148-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51148-1-AP (HYAL2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 51148-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51148-1-AP (HYAL2 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 51148-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 51148-1-AP (HYAL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, humanes Herzgewebe, humanes Lungengewebe, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

51148-1-AP bindet in WB, IHC, IF/ICC, ELISA HYAL2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HYAL2 fusion protein Ag0582
• Vollständiger Name: hyaluronoglucosaminidase 2
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC000692
• Gene symbol: HYAL2
• Gene ID (NCBI): 8692
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hyaluronic acid (HA) is a glycosaminoglycan that is believed to have numerous important biologic functions, including modulation of cell proliferation, migration, and differentiation, as well as the regulation of extracellular water and protein homeostasis. It is also an integral structural component of cartilage and other tissues and acts as a lubricant in joints. Hyaluronidases are a family of enzymes that catalyse the degradation of HA. In humans, there are five functional hyaluronidases: HYAL1, HYAL2, HYAL3, HYAL4 and HYAL5 (also known as SPAM1 or PH-20); plus a pseudogene, HYAL6 (also known as HYALP1). HYAL2 is present in many tissues except the adult brain (PMID: 11731268). HYAL2 can be exposed to cell surfaces that bind to the plasma membrane via GPI anchors (PMID: 11731268).

Publikationen

Species	Application	Title
human	WB,IHC	Dev Cell; High-Molecular-Weight Hyaluronan Is a Hippo Pathway Ligand Directing Cell Density-Dependent Growth Inhibition via PAR1b.; Authors - Takuya Ooki ; KD Validated