HYAL4 Polyklonaler Antikörper

HYAL4 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18139-1-AP

Synonyme

Hyal 4, HYAL4, hyaluronoglucosaminidase 4

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Galerie der Validierungsdaten

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  WB analysis using 18139-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18139-1-AP (HYAL4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 18139-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18139-1-AP (HYAL4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 18139-1-AP

  IP Result of anti-HYAL4 (IP:18139-1-AP, 4ug; Detection:18139-1-AP 1:300) with mouse testis tissue lysate 4000ug.

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  IHC staining of human testis using 18139-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18139-1-AP (HYAL4 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 18139-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18139-1-AP (HYAL4 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 18139-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18139-1-AP (HYAL4 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 18139-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18139-1-AP (HYAL4 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, Maushodengewebe, NIH/3T3-Zellen, Rattenhodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

18139-1-AP bindet in WB, IP, IHC, ELISA HYAL4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HYAL4 fusion protein Ag12766
• Vollständiger Name: hyaluronoglucosaminidase 4
• Berechnetes Molekulargewicht: 481 aa, 54 kDa
• Beobachtetes Molekulargewicht: 60-65 kDa
• GenBank-Zugangsnummer: BC104788
• Gene symbol: HYAL4
• Gene ID (NCBI): 23553
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hyaluronic acid (HA), a glycosaminoglycan that is ubiquitously present in the extracellular space of higher animals, maintains matrix structure and controls cellular functions such as proliferation, differentiation, and locomotion. Hyal enzymes that catabolize HA, are involved in development and tumorigenesis.HYAL4, a member of the Hyal family, is a chondroitin sulfate (CS)-specific endo-β-acetylgalactosaminidase. The expression of hHYAL4 mRNA is not ubiquitous but restricted to placenta, skeletal muscle, and testis (PMID: 23086929). This polyclonal antibody raised against 29-460aa of human HYAL4. The apparent molecular weight is larger than the calculated molecular weight of 54 kDa, which is likely due to posttranslational modification, presumably by glycosylation (PMID: 19889881).

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