Haptoglobin Polyklonaler Antikörper

Haptoglobin Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16665-1-PBS

Synonyme

HP, BP, HAPT, HP2 ALPHA 2, HPA1S

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  WB analysis using 16665-1-AP (same clone as 16665-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16665-1-AP (Haptoglobin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16665-1-PBS in a different storage buffer formulation.

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  WB analysis using 16665-1-AP (same clone as 16665-1-PBS)

  Human plasma were subjected to SDS PAGE followed by western blot with 16665-1-AP (Haptoglobin antibody) at dilution of 1:18000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16665-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 16665-1-AP (same clone as 16665-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16665-1-AP (Haptoglobin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16665-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 16665-1-AP (same clone as 16665-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16665-1-AP (Haptoglobin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16665-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 16665-1-AP (same clone as 16665-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16665-1-AP (Haptoglobin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16665-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 16665-1-AP (same clone as 16665-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16665-1-AP (Haptoglobin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16665-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 16665-1-AP (same clone as 16665-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16665-1-AP (Haptoglobin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16665-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 16665-1-AP (same clone as 16665-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16665-1-AP (Haptoglobin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16665-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16665-1-PBS bindet in WB, IHC, Indirect ELISA Haptoglobin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Haptoglobin fusion protein Ag10143
• Vollständiger Name: haptoglobin
• Berechnetes Molekulargewicht: 281aa,31 kDa; 228aa,25 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC058031
• Gene symbol: HP
• Gene ID (NCBI): 3240
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HP(Haptoglobin) is also named as zonulin and belongs to the peptidase S1 family. HP, a plasma glycoprotein that binds free hemoglobin, has a tetrameric structure of 2 alpha(16 kDa and 9 kDa) and 2 beta(40 kDa) polypeptides that are covalently associated by disulfide bonds. In most species, apart from ruminants, Hp has a molecular mass of 100 kDa, consisting of two subunits of 40 kDa and two subunits of 9 kDa, although in a few species, such as man, genetic variant of Hp forms polymers of higher mass(PMID:2361363). Recent studies of haptoglobin show that certain oligosaccharide structures predominate in different diseases. For example, a highly-fucosylated structure is found in breast cancer and ovarian cancer, highly-sialylated structures in Crohn's disease and highly branched structures in alcoholic liver disease and fucosylated haptoglobin is a good serum marker for pancreatic cancer.(PMID:16385567).