Haptoglobin Polyklonaler Antikörper

Haptoglobin Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Kaninchen, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30412-1-AP

Synonyme

HP, Zonulin, HPA1S, HP2 ALPHA 2, HAPT

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  WB analysis using 30412-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30412-1-AP (Haptoglobin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30412-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30412-1-AP (Haptoglobin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of human plasma using 30412-1-AP

  IP result of anti-Haptoglobin (IP:30412-1-AP, 4ug; Detection:30412-1-AP 1:3000) with human plasma lysate 1600 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: rabbit blood tissue, pig plasma, rabbit plasma
• Erfolgreiche IP: human plasma

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30412-1-AP bindet in WB, IP, ELISA Haptoglobin und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: haptoglobin
• Beobachtetes Molekulargewicht: 36-45 kDa
• GenBank-Zugangsnummer: BC121125
• Gene symbol: HP
• Gene ID (NCBI): 3240
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Haptoglobin is also named as Zonulin and belongs to the peptidase S1 family. Haptoglobin is an alpha-2-glycoprotein synthesized mainly by hepatocytes consisting of both alpha and beta subunits linked by disulfide bonds forming dimers (PMID: 4018023). Haptoglobin is primarily produced in the liver, and when red cells are lysed in vivo, free hemoglobin binds to circulating haptoglobin; the hemoglobin-haptoglobin complex is then degraded by the reticuloendothelial system (PMID: 24809098). Because haptoglobin levels become depleted in the presence of large amounts of free hemoglobin, decreased haptoglobin is a marker of hemolysis. The human haptoglobin (HP) gene exists in two major allelic forms leading to three major genotypes: Hp1-1, Hp2-1, and Hp-2-2, that separate on alkaline starch gel electrophoresis due to different molecular weights (PMID: 17474882). Their properties vary in binding strength, anti-oxidative ability, and rate of clearance. Binding strength is greatest with Hp1-1, but the Hp-2 genotypes can bind a larger number of hemoglobin alpha-beta dimers. Complexes of Hp2-2 and hemoglobin have higher affinity for the CD163 receptor, the monocyte-macrophage receptor that facilitates its clearance. Neutrophils contained a large amount of highly glycosylated Hp (β-chain 45-65 kDa) synthesized in neutrophil precursors and stored in specific granules and a small amount of Hp (β-chain 39 kDa) endocytosed from plasma and stored in secretory vesicles (PMID: 16543473).