Harmonin Polyklonaler Antikörper

Harmonin Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11358-1-PBS

Synonyme

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  WB analysis using 11358-1-AP (same clone as 11358-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11358-1-AP (Harmonin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11358-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 11358-1-AP (same clone as 11358-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11358-1-AP (Harmonin antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11358-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 11358-1-AP (same clone as 11358-1-PBS)

  IP result of anti-Harmonin (IP:11358-1-AP, 4ug; Detection:11358-1-AP 1:600) with HEK-293 cells lysate 3000ug. This data was developed using the same antibody clone with 11358-1-PBS in a different storage buffer formulation.

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  IHC staining of Human Colon cancer using 11358-1-AP (same clone as 11358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 11358-1-AP (Harmonin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11358-1-PBS in a different storage buffer formulation.

• 

  IHC staining of Human Colon cancer using 11358-1-AP (same clone as 11358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 11358-1-AP (Harmonin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11358-1-PBS in a different storage buffer formulation.

• 

  IHC staining of Human Colon cancer using 11358-1-AP (same clone as 11358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 11358-1-AP (Harmonin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11358-1-PBS in a different storage buffer formulation.

• 

  IHC staining of Human Colon cancer using 11358-1-AP (same clone as 11358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 11358-1-AP (Harmonin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11358-1-PBS in a different storage buffer formulation.

• 

  IHC staining of Human Colon cancer using 11358-1-AP (same clone as 11358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 11358-1-AP (Harmonin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11358-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 11358-1-AP (same clone as 11358-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Harmonin antibody (11358-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11358-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11358-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA Harmonin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Harmonin fusion protein Ag1919
• Vollständiger Name: Usher syndrome 1C (autosomal recessive, severe)
• Berechnetes Molekulargewicht: 552 aa, 62 kDa
• Beobachtetes Molekulargewicht: 68-73 kDa
• GenBank-Zugangsnummer: BC016057
• Gene symbol: USH1C/Harmonin
• Gene ID (NCBI): 10083
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Harmonin, also known as USH1C, is a scaffold protein which interacts with other USH1 proteins (USH1B/myosin VIIa and USH1G/sans) to form complex and is implicated in proper development, morphogenesis, and structural integrity of the hair cell mechanoreceptor. Mutations of Harmonin cause Usher syndrome type 1 (congenital deafness and balance disorder, and early-onset sight loss).