Hemoglobin Alpha Polyklonaler Antikörper

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Hemoglobin Alpha Polyklonal Antikörper für WB, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14537-1-PBS

Synonyme

HBA1, Hemoglobin alpha chain, Hemoglobin a, HBA2, Alpha-globin

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  WB analysis using 14537-1-AP (same clone as 14537-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14537-1-AP (Hemoglobin Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14537-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14537-1-AP (same clone as 14537-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14537-1-AP (Hemoglobin Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14537-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 14537-1-AP (same clone as 14537-1-PBS)

  IP result of anti-Hemoglobin Alpha (IP:14537-1-AP, 4ug; Detection:14537-1-AP 1:500) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 14537-1-PBS in a different storage buffer formulation.

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  IF Staining of human appendicitis using 14537-1-AP (same clone as 14537-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Hemoglobin Alpha antibody (14537-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14537-1-PBS in a different storage buffer formulation.

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  IF Staining of human placenta using 14537-1-AP (same clone as 14537-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Hemoglobin Alpha antibody (14537-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14537-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14537-1-PBS bindet in WB, IF-P, IP, ELISA Hemoglobin Alpha und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Hemoglobin Alpha fusion protein Ag6040
• Vollständiger Name: hemoglobin, alpha 1
• Berechnetes Molekulargewicht: 15 kDa
• Beobachtetes Molekulargewicht: 14-16 kDa, 27 kDa
• GenBank-Zugangsnummer: BC050661
• Gene symbol: HBA1
• Gene ID (NCBI): 3039
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBA1 (hemoglobin alpha chain) protein is a alpha-type chain of hemoglobin encoded by two independent genes (HBA1 and HBA2) whose coding sequences are identical. Two alpha chains coupled with two beta chains constitute the adult hemoglobin (HbA or α2β2). HBA1 is also the component of fetal hemoglobin (HbF or α2γ2). This antibody detects a major band around 14-16 kDa in the western blot analysis of heart tissue and K-562 cells. A higher band around 26-27 kDa can also be observed occasionally, which may represents the dimer form of HBA1 (PMID: 20836851). This antibody may cross-react with other hemoglobin chains.