Hemoglobin Epsilon Polyklonaler Antikörper

Hemoglobin Epsilon Polyklonal Antikörper für IF-P, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF-P, FC (Intra)

Konjugation

CoraLite®555 Fluorescent Dye

Kat-Nr. : CL555-12361

Synonyme

HBE1, Epsilon globin, Epsilon-globin, HBE, Hemoglobin epsilon chain

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  IF Staining of human placenta using CL555-12361

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CoraLite®555 Hemoglobin Epsilon antibody (CL555-12361) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of K-562 using CL555-12361

  1x10^6 K-562 cells were intracellularly stained with 0.8 ug CoraLite®555-conjugated Hemoglobin Epsilon Polyclonal antibody (CL555-12361)(red), or 0.8 ug CoraLite®555-conjugated Rabbit IgG control Rabbit PolyAb (CL555-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in FC (Intra): K-562-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL555-12361 bindet in IF-P, FC (Intra) Hemoglobin Epsilon und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Hemoglobin Epsilon fusion protein Ag3026
• Vollständiger Name: hemoglobin, epsilon 1
• Berechnetes Molekulargewicht: 147 aa, 16 kDa
• Beobachtetes Molekulargewicht: 12-16 kDa
• GenBank-Zugangsnummer: BC015537
• Gene symbol: HBE1
• Gene ID (NCBI): 3046
• Konjugation: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBE1 (hemoglobin epsilon chain) is a beta-type chain of hemoglobin predominantly expressed during the embryonic stage (21321359). The HBE1 has been regarded as the best marker for fetal nucleated red blood cells (NRBCs) . This antibody can be used to label and isolate fetal cells from maternal blood and may be useful in prenatal diagnosis (15906407).