Hemopexin Monoklonaler Antikörper

Hemopexin Monoklonal Antikörper für WB, IHC, IF-P, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Ratte

Anwendung

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3A9D6

Kat-Nr. : 66479-1-PBS

Synonyme

HPX, Beta-1B-glycoprotein, Beta 1B glycoprotein, 3A9D6

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  WB analysis of human plasma using 66479-1-Ig (same clone as 66479-1-PBS)

  0.7 μL human plasma was subjected to SDS PAGE followed by western blot with 66479-1-Ig (Hemopexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

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  WB analysis of pig plasma using 66479-1-Ig (same clone as 66479-1-PBS)

  0.7 μL pig plasma was subjected to SDS PAGE followed by western blot with 66479-1-Ig (Hemopexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

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  WB analysis of rat serum using 66479-1-Ig (same clone as 66479-1-PBS)

  0.7 μL rat serum was subjected to SDS PAGE followed by western blot with 66479-1-Ig (Hemopexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 66479-1-Ig (same clone as 66479-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66479-1-Ig (Hemopexin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66479-1-Ig (same clone as 66479-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66479-1-Ig (Hemopexin antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66479-1-Ig (same clone as 66479-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66479-1-Ig (Hemopexin antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66479-1-Ig (same clone as 66479-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66479-1-Ig (Hemopexin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 66479-1-Ig (same clone as 66479-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Hemopexin antibody (66479-1-Ig, Clone: 3A9D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 66479-1-Ig (same clone as 66479-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human Hemopexin (66479-1-Ig, Clone:3A9D6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66479-1-PBS bindet in WB, IHC, IF-P, FC (Intra), Indirect ELISA Hemopexin und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Hemopexin fusion protein Ag8533
• Vollständiger Name: hemopexin
• Berechnetes Molekulargewicht: 254 aa, 29 kDa
• Beobachtetes Molekulargewicht: 65-75 kDa
• GenBank-Zugangsnummer: BC005395
• Gene symbol: Hemopexin
• Gene ID (NCBI): 3263
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hemopexin (HPX) is the plasma protein responsible for scavenging heme, thus preventing heme-mediated oxidative stress and heme-bound iron loss. In addition, hemopexin blocks heme activation of immune receptors and vascular inflammatory processes. It is mainly expressed in liver, the synthesis of which is induced after inflammation. Alterations of plasma hemopexin level have been linked to disorders like atherosclerosis and inflammatory diseases.