Hemopexin Rekombinanter Antikörper

Hemopexin Rekombinant Antikörper für WB, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250345E5

Kat-Nr. : 86395-3-PBS

Synonyme

HPX, Beta 1B glycoprotein, Beta-1B-glycoprotein

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  WB analysis of human plasma using 86395-3-RR (same clone as 86395-3-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 86395-3-RR (HPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86395-3-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 86395-3-RR (same clone as 86395-3-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 86395-3-RR (HPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86395-3-PBS in a different storage buffer formulation.

• 

  FC experiment of Transfected CHO using 86395-3-RR (same clone as 86395-3-PBS)

  1x10^6 CHO cells or Transfected CHO cells were intracellularly stained with 0.25 ug Hemopexin Recombinant monoclonal antibody (86395-3-RR, Clone:250345E5) and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 86395-3-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

86395-3-PBS bindet in WB, FC (Intra), Indirect ELISA Hemopexin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Hemopexin fusion protein Eg3082
• Vollständiger Name: hemopexin
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 65-75 kDa
• GenBank-Zugangsnummer: NM_000613.2
• Gene symbol: Hemopexin
• Gene ID (NCBI): 3263
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hemopexin (HPX) is the plasma protein responsible for scavenging heme, thus preventing heme-mediated oxidative stress and heme-bound iron loss. In addition, hemopexin blocks heme activation of immune receptors and vascular inflammatory processes. It is mainly expressed in liver, the synthesis of which is induced after inflammation. Alterations of plasma hemopexin level have been linked to disorders like atherosclerosis and inflammatory diseases.