Hemopexin Rekombinanter Antikörper

Hemopexin Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

250345E5

Kat-Nr. : 86395-3-RR

Synonyme

HPX, Beta 1B glycoprotein, Beta-1B-glycoprotein

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  WB analysis of human plasma using 86395-3-RR

  human plasma were subjected to SDS PAGE followed by western blot with 86395-3-RR (HPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 86395-3-RR

  human placenta tissue were subjected to SDS PAGE followed by western blot with 86395-3-RR (HPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of Transfected CHO using 86395-3-RR

  1x10^6 CHO cells or Transfected CHO cells were intracellularly stained with 0.25 ug Hemopexin Recombinant monoclonal antibody (86395-3-RR, Clone:250345E5) and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, human plasma
• Erfolgreiche Detektion in FC (Intra): Transfected CHO

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86395-3-RR bindet in WB, FC (Intra), ELISA Hemopexin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Hemopexin fusion protein Eg3082
• Vollständiger Name: hemopexin
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 65-75 kDa
• GenBank-Zugangsnummer: NM_000613.2
• Gene symbol: Hemopexin
• Gene ID (NCBI): 3263
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hemopexin (HPX) is the plasma protein responsible for scavenging heme, thus preventing heme-mediated oxidative stress and heme-bound iron loss. In addition, hemopexin blocks heme activation of immune receptors and vascular inflammatory processes. It is mainly expressed in liver, the synthesis of which is induced after inflammation. Alterations of plasma hemopexin level have been linked to disorders like atherosclerosis and inflammatory diseases.