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HER2/ErbB2 Rekombinanter Antikörper

HER2/ErbB2 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241366F4

Kat-Nr. : 84128-5-PBS

Synonyme

Her2 / ErbB2, Her2, Erbb2, EC:2.7.10.1, CD340

Filter:
  • Western Blot (WB) analysis of 4T1 cells using HER2/ErbB2 Recombinant antibody (84128-5-RR)

    WB analysis of 4T1 using 84128-5-RR (same clone as 84128-5-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 84128-5-RR (Her2 / ErbB2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84128-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of 4T1 cells using HER2/ErbB2 Recombinant antibody (84128-5-RR)

    IF Staining of 4T1 using 84128-5-RR (same clone as 84128-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed 4T1 cells using Her2 / ErbB2 antibody (84128-5-RR, Clone: 241366F4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84128-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84128-5-RR

    Affinity and Kinetic Characterization of 84128-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84128-5-RR against Mouse Her2 / ErbB2 were performed. The affinity constant is 0.356 nM.

Geprüfte Anwendungen

Produktinformation

84128-5-PBS bindet in WB, IF/ICC, Indirect ELISA HER2/ErbB2 und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Fusionsprotein
Vollständiger Name v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
Berechnetes Molekulargewicht139 kDa
Beobachtetes Molekulargewicht185 kDa
GenBank-ZugangsnummerNM_001003817.1
Gene symbol Erbb2
Gene ID (NCBI) 13866
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Her2, or ErbB2, is a member of the EGF receptor family of receptor tyrosine kinases. It has no ligand-binding domain of its own. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signaling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase.