HER2/ErbB2 Rekombinanter Antikörper

HER2/ErbB2 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241366F4

Kat-Nr. : 84128-5-RR

Synonyme

Her2 / ErbB2, Her2, Erbb2, EC:2.7.10.1, CD340

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  WB analysis of 4T1 using 84128-5-RR

  4T1 cells were subjected to SDS PAGE followed by western blot with 84128-5-RR (Her2 / ErbB2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of 4T1 using 84128-5-RR

  Immunofluorescent analysis of (4% PFA) fixed 4T1 cells using Her2 / ErbB2 antibody (84128-5-RR, Clone: 241366F4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84128-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84128-5-RR against Mouse Her2 / ErbB2 were performed. The affinity constant is 0.356 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: 4T1-Zellen
• Erfolgreiche Detektion in IF/ICC: 4T1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84128-5-RR bindet in WB, IF/ICC, ELISA HER2/ErbB2 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
• Berechnetes Molekulargewicht: 139 kDa
• Beobachtetes Molekulargewicht: 185 kDa
• GenBank-Zugangsnummer: NM_001003817.1
• Gene symbol: Erbb2
• Gene ID (NCBI): 13866
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Her2, or ErbB2, is a member of the EGF receptor family of receptor tyrosine kinases. It has no ligand-binding domain of its own. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signaling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase.