Hexokinase 1 Monoklonaler Antikörper

Hexokinase 1 Monoklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2B4F9

Kat-Nr. : 68419-1-PBS

Synonyme

HK1, Hexokinase-1, Hexokinase1, Hexokinase type I, EC:2.7.1.1

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  WB analysis using 68419-1-Ig (same clone as 68419-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68419-1-Ig (HK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68419-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 68419-1-Ig (same clone as 68419-1-PBS)

  IP result of anti-Hexokinase 1 (IP:68419-1-Ig, 4ug; Detection:68419-1-Ig 1:2000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 68419-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68419-1-Ig (same clone as 68419-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68419-1-Ig (Hexokinase 1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68419-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68419-1-Ig (same clone as 68419-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Hexokinase 1 antibody (68419-1-Ig, Clone: 2B4F9 ) at dilution of 1:2049 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004). This data was developed using the same antibody clone with 68419-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68419-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA Hexokinase 1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Hexokinase 1 fusion protein Ag8015
• Vollständiger Name: hexokinase 1
• Berechnetes Molekulargewicht: 102 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC008730
• Gene symbol: Hexokinase 1
• Gene ID (NCBI): 3098
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The hexokinases (HKs) catalyze the first obligatory step in glucose metabolism to generate glucose-6-phosphate. This not only furthers glucose entry by maintaining the concentration gradient for facilitated glucose influx but also provides the first intermediate for essentially all major pathways using glucose. There are four conventional HK isoforms, HK1/2/3/4, encoded by four different genes. Most adult tissues express only HK1. Muscle and adipose tissue use HK2 for glycolysis, liver, and pancreatic beta cells express HK4 (also called glucokinase) and do not express HK1 or HK2. (PMID: 31434645, PMID: 31848318)