Hexokinase 2 Rekombinanter Antikörper

Hexokinase 2 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250190C10

Kat-Nr. : 85875-1-RR

Synonyme

HK2, EC:2.7.1.1, Hexokinase type II, hexokinase2, Hexokinase-2

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  WB analysis using 85875-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85875-1-RR (Hexokinase 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85875-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85875-1-RR (Hexokinase 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 85875-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 85875-1-RR (Hexokinase 2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 85875-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 85875-1-RR (Hexokinase 2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 85875-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85875-1-RR against Human Hexokinase 2 were performed. The affinity constant is 0.302 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HCT 116-Zellen, Jurkat-Zellen, MCF-7-Zellen, Mausherzgewebe, Maus-Skelettmuskelgewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85875-1-RR bindet in WB, IHC, ELISA Hexokinase 2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Hexokinase 2 fusion protein Ag16895
• Vollständiger Name: hexokinase 2
• Berechnetes Molekulargewicht: 102 kDa
• Beobachtetes Molekulargewicht: 102-120 kDa
• GenBank-Zugangsnummer: BC021116
• Gene symbol: Hexokinase 2
• Gene ID (NCBI): 3099
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hexokinase 2 (HK2), a rate-limiting enzyme in the first step of glycolysis pathway, expresses at high level in cancer cells compared with normal cells. HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic process. HK1 is constitutively expressed in most mammalian adult tissues. HK2, however, although is abundantly expressed in embryonic tissues, is expressed at high levels only in limited number of adult tissues such as adipose, skeletal, and cardiac muscles. (PMID: 29305912, PMID: 28427443, PMID: 23911236).