HGF Rekombinanter Antikörper

HGF Rekombinant Antikörper für Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242476H8

Kat-Nr. : 85014-3-PBS

Synonyme

Hepatocyte growth factor, Hepatocyte growth factor alpha chain, Hepatocyte growth factor beta chain, Hepatopoietin-A, Scatter factor

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  Sandwich ELISA standard curve of MP01760-3

  Sandwich ELISA standard curve of MP01760-3, Mouse HGF Recombinant Matched Antibody Pair - PBS only. 85014-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0675. 85014-3-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL.

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  Sample test of MP01760-3

  The mean HGF concentration was determined to be 2,919.6 pg/mL in mouse kidney tissue extract based on a 3.1 mg/mL extract load and 13,658.8 pg/mL in mouse liver tissue extract based on a 3.7 mg/mL extract load.

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  Sample test of MP01760-3

  Serum of six mice was measured. The HGF concentration of detected samples was determined to be 7,505.4 pg/mL with a range of 5,590.3-9,962.8 pg/mL.

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  Sample test of MP01760-3

  Serum of six rats was measured. The HGF concentration of detected samples was determined to be 40,922.2 pg/mL with a range of 21,345.0-50,183.1 pg/mL.

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  Sample test of MP01760-3

  3T3-L1 cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for mouse HGF, and measured 3,491.8 pg/mL.

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Geprüfte Anwendungen

Produktinformation

85014-3-PBS bindet in Sandwich ELISA, Indirect ELISA, Sample test HGF und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HGF fusion protein Eg0675
• Vollständiger Name: hepatocyte growth factor
• Berechnetes Molekulargewicht: 83KD
• GenBank-Zugangsnummer: NM_001289458.1
• Gene symbol: Hgf
• Gene ID (NCBI): 15234
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hepatocyte growth factor (HGF) is the most potent mitogen of mature hepatocytes in primary culture. HGF is derived from a biologically inactive single chain precursor of 728 amino acids (pro-HGF) localized mostly on the cell surface and in the extracellular matrix. The mature form produced following proteolytic cleavage is composed of a 69-kDa α-subunit (containing four kringle domains) and the 34 kDa β-subunit, similar to the catalytic domain of serine proteases, but with amino acid substitutions in the active site. HGF is a pleiotropic cytokine which exerts a variety of effects on several cells, being involved in the regulation of many biological processes, such as inflammation, tissue repair, morphogenesis, angiogenesis, tumour propagation, immunomodulation of viral infections and cardio-metabolic activities.