Hif1a Rekombinanter Antikörper

Hif1a Rekombinant Antikörper für IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230101B3

Kat-Nr. : 82989-6-PBS

Synonyme

ARNT-interacting protein, HIF-1 alpha, Hif-1a, HIF1alpha, HIF-1-alpha

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  IHC staining of mouse kidney using 82989-6-RR (same clone as 82989-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82989-6-RR (Hif1a antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82989-6-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 82989-6-RR (same clone as 82989-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82989-6-RR (Hif1a antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82989-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82989-6-RR

  Biolayer interferometry (BLl) kinetic assays of 82989-6-RR against Mouse Hif1a were performed. The affinity constant is 0.163 nM.

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Geprüfte Anwendungen

Produktinformation

82989-6-PBS bindet in IHC, Indirect ELISA Hif1a und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Hif1a fusion protein Ag33620
• Vollständiger Name: hypoxia inducible factor 1, alpha subunit
• Berechnetes Molekulargewicht: 93 kDa
• GenBank-Zugangsnummer: NM_001313919
• Gene symbol: Hif1a
• Gene ID (NCBI): 15251
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia