Histone H1.0 Rekombinanter Antikörper

Histone H1.0 Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

241147D2

Kat-Nr. : 83993-1-RR

Synonyme

H1F0, H1FV, H1-0, H10, H1 histone family, member 0

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  WB analysis using 83993-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83993-1-RR (Histone H1.0 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 83993-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83993-1-RR (Histone H1.0 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of BxPC-3 using 83993-1-RR

  1x10^6 BxPC-3 cells were intracellularly stained with 0.25 ug Histone H1.0 Recombinant antibody (83993-1-RR, Clone:241147D2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, C6-Zellen, Maushodengewebe, NIH/3T3-Zellen, Rattenmilzgewebe
• Erfolgreiche Detektion in FC (Intra): BxPC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83993-1-RR bindet in WB, FC (Intra), ELISA Histone H1.0 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Histone H1.0 fusion protein Ag9982
• Vollständiger Name: H1 histone family, member 0
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC000145
• Gene symbol: Histone H1.0
• Gene ID (NCBI): 3005
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Linker histones are involved in forming higher order structure in chromatin and maintaining overall chromatin compaction. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.Histone H1.0 (H1F0,H1FV) is a linker histone that is widely expressed in many tissues and almost all vertebrates, unlike some other linker histones. The observed molecular weight of H1F0 is about 32 kDa.