Histone H2A.X Monoklonaler Antikörper

Histone H2A.X Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

3E11B2

Kat-Nr. : 68888-1-PBS

Synonyme

H2AFX, 3E11B2, H2A histone family, member X, H2A.X, H2AX

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  WB analysis using 68888-1-Ig (same clone as 68888-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68888-1-Ig (Histone H2A.X antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68888-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 68888-1-Ig (same clone as 68888-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer slide using 68888-1-Ig (Histone H2A.X antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68888-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 68888-1-Ig (same clone as 68888-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68888-1-Ig (Histone H2A.X antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68888-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 68888-1-Ig (same clone as 68888-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer slide using 68888-1-Ig (Histone H2A.X antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68888-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68888-1-Ig (same clone as 68888-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Histone H2A.X antibody (68888-1-Ig, Clone: 3E11B2 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68888-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68888-1-Ig (same clone as 68888-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Histone H2A.X antibody (68888-1-Ig, Clone: 3E11B2 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68888-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 68888-1-Ig (same clone as 68888-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.1 ug Histone H2A.X Monoclonal antibody (68888-1-Ig, Clone:3E11B2) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005)(red), or 0.1 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100. This data was developed using the same antibody clone with 68888-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68888-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), ELISA Histone H2A.X und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: H2A histone family, member X
• Berechnetes Molekulargewicht: 15 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC013416
• Gene symbol: Histone H2A.X
• Gene ID (NCBI): 3014
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histone H2A.X belongs to the histone H2A family, which is synthesized in G1 and S phases. It is involved in nucleosomal organization of chromatin together with other histone proteins and is especially important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 (to form gamma-H2AFX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, which promotes DNA repair and maintains genomic stability. The calculated molecular weight of H2AX is 15 kDa, but the ubiquitinated H2A.X is about 22 kDa.