Histone H2A.X Rekombinanter Antikörper

Histone H2A.X Rekombinant Antikörper für WB, IHC, Indirect ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

250032F5

Kat-Nr. : 83307-3-PBS

Synonyme

H2AFX, H2A histone family, member X, H2A.X, H2AX, Histone H2A

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  WB analysis using 83307-3-RR (same clone as 83307-3-PBS)

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 83307-3-RR (Histone H2A.X antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83307-3-PBS in a different storage buffer formulation.

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  IHC staining of human kidney(renal cell) Cancer using 83307-3-RR (same clone as 83307-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney(renal cell) Cancer slide using 83307-3-RR (Histone H2A.X antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83307-3-PBS in a different storage buffer formulation.

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  IHC staining of human kidney(renal cell) Cancer using 83307-3-RR (same clone as 83307-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney(renal cell) Cancer slide using 83307-3-RR (Histone H2A.X antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83307-3-PBS in a different storage buffer formulation.

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  ChIP experiment of HEK-293 using 83307-3-RR (same clone as 83307-3-PBS)

  Chromatin was prepared from HEK-293 cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Histone H2A.X (83307-3-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 83307-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83307-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83307-3-RR against Human Histone H2A.X were performed. The affinity constant is 3.46 nM.

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Geprüfte Anwendungen

Produktinformation

83307-3-PBS bindet in WB, IHC, Indirect ELISA, ChIP-qPCR Histone H2A.X und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Histone H2A.X fusion protein Ag1305
• Vollständiger Name: H2A histone family, member X
• Berechnetes Molekulargewicht: 15 kDa
• Beobachtetes Molekulargewicht: 15-18 kDa, 22 kDa
• GenBank-Zugangsnummer: BC013416
• Gene symbol: Histone H2A.X
• Gene ID (NCBI): 3014
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histone H2A.X belongs to the histone H2A family, which is synthesized in G1 and S phase. It is involved in nucleosomal organization of chromatin together with other histone proteins, and is specially important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 (to form gamma-H2AFX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, which promotes DNA repair and maintains genomic stability. The calculated molecular weight of H2AX is 15 kDa, but the ubiquitinated H2A.X is about 22 kDa.