Histone H2B Polyklonaler Antikörper

Histone H2B Polyklonal Antikörper für WB, IHC, IF, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15857-1-PBS

Synonyme

HIST2H2BE, GL105, H2B, H2B.1, H2B/q

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  WB analysis using 15857-1-AP (same clone as 15857-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15857-1-AP (Histone H2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

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  WB analysis using 15857-1-AP (same clone as 15857-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15857-1-AP (Histone H2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse pancreas using 15857-1-AP (same clone as 15857-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15857-1-AP (Histone H2B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

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  WB analysis of rat kidney using 15857-1-AP (same clone as 15857-1-PBS)

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 15857-1-AP (Histone H2B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 15857-1-AP (same clone as 15857-1-PBS)

  IP result of anti-Histone H2B (IP:15857-1-AP, 4ug; Detection:15857-1-AP 1:500) with HEK-293 cells lysate 2000ug. This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 15857-1-AP (same clone as 15857-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15857-1-AP (Histone H2B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 15857-1-AP (same clone as 15857-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15857-1-AP (Histone H2B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15857-1-AP (same clone as 15857-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15857-1-AP (Histone H2B Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15857-1-AP (same clone as 15857-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15857-1-AP (Histone H2B Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

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  IF Staining using 15857-1-AP (same clone as 15857-1-PBS)

  Mouse tumour sections were labelled with H2B (15857-1-AP, red), Dendra2 (green), and DAPI. The image was credited by Dr. Hae Lin Jang from Harvard Medical School. This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15857-1-PBS bindet in WB, IHC, IF, IP, Indirect ELISA Histone H2B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Histone H2B fusion protein Ag7811
• Vollständiger Name: histone cluster 2, H2be
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 14-17 kDa
• GenBank-Zugangsnummer: BC005827
• Gene symbol: Histone H2B
• Gene ID (NCBI): 8349
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histone H2B is a core component of the nucleosome, the fundamental unit of chromatin. It forms a dimer with Histone H2A, and two H2A-H2B dimers combine with a (H3-H4)₂ tetramer to create the histone octamer. The primary function of this structure is to package DNA into a compact and manageable form. However, H2B is also subject to various post-translational modifications (PTMs), such as ubiquitination, which serve as epigenetic marks to regulate gene expression by altering chromatin accessibility.