Histone H2B Polyklonaler Antikörper

Histone H2B Polyklonal Antikörper für WB, IF/ICC, ChIP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ChIP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27740-1-PBS

Synonyme

H2B3B, HIST3H2BB, H2B-clustered histone 26, H2BC26, H2B.U histone 1

---

• 

  WB analysis of HEK-293T using 27740-1-AP (same clone as 27740-1-PBS)

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 27740-1-AP (HIST3H2BB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27740-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 27740-1-AP (same clone as 27740-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27740-1-AP (HIST3H2BB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27740-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 27740-1-AP (same clone as 27740-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 27740-1-AP (HIST3H2BB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27740-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 27740-1-AP (same clone as 27740-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using Histone H2B antibody (27740-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27740-1-PBS in a different storage buffer formulation.

• 

  ChIP experiment of HEK-293 using 27740-1-AP (same clone as 27740-1-PBS)

  Chromatin was prepared from HEK-293 cells, cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 18 µg of cross-linked chromatin, 5 µg of Histone H2B Antibody (27740-1-AP) or 5 ug of Normal Rabbit IgG (30000-0-AP), and 30 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real time PCR. Primers are located in the first kb of the transcribed region. This data was developed using the same antibody clone with 27740-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

27740-1-PBS bindet in WB, IF/ICC, ChIP, Indirect ELISA Histone H2B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Histone H2B fusion protein Ag26901
• Vollständiger Name: histone cluster 3, H2bb
• Beobachtetes Molekulargewicht: 15-20 kDa
• GenBank-Zugangsnummer: BC100855
• Gene symbol: Histone H2B
• Gene ID (NCBI): 128312
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. HIST3H2BB is a replication-dependent histone that is a member of the histone H2B family.