Histone H3.3 Polyklonaler Antikörper

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• KD/KO Validated

Histone H3.3 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13754-1-PBS

Synonyme

H3F3A, HistoneH3.3, Histone H3, H3-3B, H3-3A

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  WB analysis using 13754-1-AP (same clone as 13754-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13754-1-AP (Histone H3.3 antibody) at dilution of 1:1000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 13754-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 13754-1-AP (same clone as 13754-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13754-1-AP (Histone H3.3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13754-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse lung using 13754-1-AP (same clone as 13754-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13754-1-AP (Histone H3.3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13754-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 13754-1-AP (same clone as 13754-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13754-1-AP (H3F3A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13754-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 13754-1-AP (same clone as 13754-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13754-1-AP (H3F3A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13754-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse thymus using 13754-1-AP (same clone as 13754-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13754-1-AP (H3F3A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13754-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 13754-1-AP (same clone as 13754-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13754-1-AP (Histone H3.3 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13754-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 13754-1-AP (same clone as 13754-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Histone H3.3 antibody (13754-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 13754-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13754-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Histone H3.3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Histone H3.3 fusion protein Ag4696
• Vollständiger Name: H3 histone, family 3A
• Berechnetes Molekulargewicht: 136 aa, 15 kDa
• Beobachtetes Molekulargewicht: 15-17 kDa
• GenBank-Zugangsnummer: BC038989
• Gene symbol: Histone H3
• Gene ID (NCBI): 3020
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histones are the basic nuclear proteins responsible for the nucleosome structure of the chromosomal fiber. Five classes of histone genes have been reported, some of which are expressed only during S phase, while others are replication independent. The latter are referred to as replacement histones and are expressed in quiescent or terminally differentiated cells. [PMID: 8586426] Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. H3F3A is one replacement histone [PMID:16258499].