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Histone H3.4 Polyklonaler Antikörper

Histone H3.4 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18064-1-PBS

Synonyme

HIST3H3, H3.4, H3-4, H3FT, H3t

Filter:
  • Western Blot (WB) analysis of various lysates using Histone H3.4 Polyclonal antibody (18064-1-AP)

    WB analysis using 18064-1-AP (same clone as 18064-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18064-1-AP (Histone H3.4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18064-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using Histone H3.4 Polyclonal antibody (18064-1-AP)

    IHC staining of mouse testis using 18064-1-AP (same clone as 18064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18064-1-AP (Histone H3.4 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18064-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using Histone H3.4 Polyclonal antibody (18064-1-AP)

    IHC staining of mouse testis using 18064-1-AP (same clone as 18064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18064-1-AP (Histone H3.4 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18064-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using Histone H3.4 Polyclonal antibody (18064-1-AP)

    IHC staining of rat testis using 18064-1-AP (same clone as 18064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 18064-1-AP (Histone H3.4 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18064-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

18064-1-PBS bindet in WB, IHC, Indirect ELISA Histone H3.4 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Histone H3.4 fusion protein Ag12671
Vollständiger Name histone cluster 3, H3
Berechnetes Molekulargewicht 136 aa, 16 kDa
Beobachtetes Molekulargewicht16 kDa
GenBank-ZugangsnummerBC101837
Gene symbol Histone H3.4
Gene ID (NCBI) 8290
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HIST3H3, also known as H3.1t and H3.4, is a histone H3 protein variant that bears the H3.1 variant hallmark cysteine 96 but includes three extra amino acid substitutions. H3.1t was found to be expressed in primary human spermatocytes at the pachytene stage, similar to the human testis-specific histone H1t variant, where it appears therefore to take part in the process of spermatogenesis (PMID: 21782046).