Histone H3 Monoklonaler Antikörper
Histone H3 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Wirt / Isotyp
Maus / IgG2b
Getestete Reaktivität
hamster, Hausschwein, Huhn, human, Hund, hund, Kaninchen, Maus, Ratte, wheat, Zebrafisch
Anwendung
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Konjugation
Unkonjugiert
CloneNo.
1A2A3
Kat-Nr. : 68345-1-Ig
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | LNCaP-Zellen, Hühnerhirngewebe, CHO-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, MDCK-Zellen, NIH/3T3-Zellen, Zebrafisch |
Erfolgreiche IP | HeLa-Zellen |
Erfolgreiche Detektion in IHC | Maushodengewebe, humanes Kolonkarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF/ICC | MCF-7-Zellen, A431-Zellen, HeLa-Zellen |
Erfolgreiche Detektion in FC (Intra) | HepG2-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:500-1:2000 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:500-1:2000 |
Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 58 publications below |
IF | See 3 publications below |
Produktinformation
68345-1-Ig bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA Histone H3 und zeigt Reaktivität mit hamster, Hausschwein, Huhn, human, Hund, hund, Kaninchen, Maus, Ratten, wheat, Zebrafisch
Getestete Reaktivität | hamster, Hausschwein, Huhn, human, Hund, hund, Kaninchen, Maus, Ratte, wheat, Zebrafisch |
In Publikationen genannte Reaktivität | human, Maus, Ratte |
Wirt / Isotyp | Maus / IgG2b |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | Peptid |
Vollständiger Name | histone cluster 1, H3a |
Beobachtetes Molekulargewicht | 15-17 kDa |
GenBank-Zugangsnummer | BC066245 |
Gene symbol | HIST1H3A |
Gene ID (NCBI) | 8350 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-A-Reinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for Histone H3 antibody 68345-1-Ig | Protokoll herunterladen |
IHC protocol for Histone H3 antibody 68345-1-Ig | Protokoll herunterladenl |
IF protocol for Histone H3 antibody 68345-1-Ig | Protokoll herunterladen |
IP protocol for Histone H3 antibody 68345-1-Ig | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Mol Cancer Exosomal circ_0006896 promotes AML progression via interaction with HDAC1 and restriction of antitumor immunity | ||
Nucleic Acids Res The origin recognition complex requires chromatin tethering by a hypervariable intrinsically disordered region that is functionally conserved from sponge to man | ||
Redox Biol Deficiency of S100 calcium binding protein A9 attenuates vascular dysfunction in aged mice | ||
Environ Pollut Chronic exposure to polystyrene microplastics induced LHR reduction and decreased testosterone levels through NF-κB pathway | ||
Biochim Biophys Acta Mol Basis Dis ARL11 knockdown alleviates spinal cord injury by inhibiting neuroinflammation and M1 activation of microglia in mice | ||
Ecotoxicol Environ Saf Role of the USP7/FOXO3A axis in environmentally relevant doses of arsenic-induced lung carcinogenesis: Insights from bioinformatics analysis and model of human epithelial cell malignant transformation |