Histone H3 Monoklonaler Antikörper

Histone H3 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

hamster, Hausschwein, Huhn, human, Hund, hund, Kaninchen, Maus, Ratte, wheat, Zebrafisch

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(61)

CloneNo.

1A2A3

Kat-Nr. : 68345-1-Ig

Synonyme

HIST1H3A, 1A2A3, Histone H 3, HistoneH3, Histone-H3



Geprüfte Anwendungen

Erfolgreiche Detektion in WBLNCaP-Zellen, Hühnerhirngewebe, CHO-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, MDCK-Zellen, NIH/3T3-Zellen, Zebrafisch
Erfolgreiche IPHeLa-Zellen
Erfolgreiche Detektion in IHCMaushodengewebe, humanes Kolonkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCMCF-7-Zellen, A431-Zellen, HeLa-Zellen
Erfolgreiche Detektion in FC (Intra)HepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:500-1:2000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:500-1:2000
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

68345-1-Ig bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA Histone H3 und zeigt Reaktivität mit hamster, Hausschwein, Huhn, human, Hund, hund, Kaninchen, Maus, Ratten, wheat, Zebrafisch

Getestete Reaktivität hamster, Hausschwein, Huhn, human, Hund, hund, Kaninchen, Maus, Ratte, wheat, Zebrafisch
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name histone cluster 1, H3a
Beobachtetes Molekulargewicht15-17 kDa
GenBank-ZugangsnummerBC066245
Gene symbol HIST1H3A
Gene ID (NCBI) 8350
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Histone H3 antibody 68345-1-IgProtokoll herunterladen
IHC protocol for Histone H3 antibody 68345-1-IgProtokoll herunterladenl
IF protocol for Histone H3 antibody 68345-1-IgProtokoll herunterladen
IP protocol for Histone H3 antibody 68345-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Mol Cancer

Exosomal circ_0006896 promotes AML progression via interaction with HDAC1 and restriction of antitumor immunity

Authors - Can Can
WB

Nucleic Acids Res

The origin recognition complex requires chromatin tethering by a hypervariable intrinsically disordered region that is functionally conserved from sponge to man

Authors - Olubu A Adiji
mouseWB

Redox Biol

Deficiency of S100 calcium binding protein A9 attenuates vascular dysfunction in aged mice

Authors - Boying Zhao
mouseWB

Environ Pollut

Chronic exposure to polystyrene microplastics induced LHR reduction and decreased testosterone levels through NF-κB pathway

Authors - Haibo Jin
mouseWB

Biochim Biophys Acta Mol Basis Dis

ARL11 knockdown alleviates spinal cord injury by inhibiting neuroinflammation and M1 activation of microglia in mice

Authors - Haocong Zhang
humanWB

Ecotoxicol Environ Saf

Role of the USP7/FOXO3A axis in environmentally relevant doses of arsenic-induced lung carcinogenesis: Insights from bioinformatics analysis and model of human epithelial cell malignant transformation

Authors - Xiaoyu Chen