Histone H3 Rekombinanter Antikörper

Histone H3 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3D23

Kat-Nr. : 82807-2-PBS

Synonyme

HIST1H3A, 3D23, Histone H 3, HistoneH3, Histone-H3

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  WB analysis using 82807-2-RR (same clone as 82807-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82807-2-RR (HIST1H3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82807-2-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 82807-2-RR (same clone as 82807-2-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 82807-2-RR (Histone H3 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82807-2-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 82807-2-RR (same clone as 82807-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82807-2-RR (Histone H3 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82807-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 82807-2-RR (same clone as 82807-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82807-2-RR (Histone H3 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82807-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

82807-2-PBS bindet in WB, IHC, Indirect ELISA Histone H3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: histone cluster 1, H3a
• Beobachtetes Molekulargewicht: 15-17 kDa
• GenBank-Zugangsnummer: BC066245
• Gene symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes.