Histone H3 Monoklonaler Antikörper

Histone H3 Monoklonal Antikörper für IF/ICC

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

hamster, Hausschwein, Huhn, human, Hund, hund, Kaninchen, Maus, Ratte, wheat, Zebrafisch

Anwendung

IF/ICC

Konjugation

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

1A2A3

Kat-Nr. : CL405-68345

Synonyme

HIST1H3A, 1A2A3, Histone H 3, HistoneH3, Histone-H3

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  IF Staining of HeLa using CL405-68345

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 405 Histone H3 antibody (CL405-68345, Clone: 1A2A3 ) at dilution of 1:200, CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL405-68345 bindet in IF/ICC Histone H3 und zeigt Reaktivität mit hamster, Hausschwein, Huhn, human, Hund, hund, Kaninchen, Maus, Ratten, wheat, Zebrafisch

• Getestete Reaktivität: hamster, Hausschwein, Huhn, human, Hund, hund, Kaninchen, Maus, Ratte, wheat, Zebrafisch
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: histone cluster 1, H3a
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC066245
• Gene symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Konjugation: CoraLite® Plus 405 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 399 nm / 422 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes.