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- KD/KO Validated
Hsc70 Monoklonaler Antikörper
Hsc70 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
human, Maus, Ratte und mehr (3)
Anwendung
WB, IHC, IF/ICC, ELISA
Konjugation
Unkonjugiert
CloneNo.
1A4B3
Kat-Nr. : 66442-1-Ig
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | LNCaP-Zellen, 4T1-Zellen, C6-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen, ROS1728-Zellen |
| Erfolgreiche Detektion in IHC | humanes Ovarialkarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF/ICC | HeLa-Zellen, HEK-293-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunhistochemie (IHC) | IHC : 1:50-1:500 |
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 9 publications below |
| IF | See 3 publications below |
Produktinformation
66442-1-Ig bindet in WB, IHC, IF/ICC, ELISA Hsc70 und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Affe, Hausschwein, Maus, Ziege |
| Wirt / Isotyp | Maus / IgG1 |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | Hsc70 fusion protein Ag17910 |
| Vollständiger Name | heat shock 70kDa protein 8 |
| Berechnetes Molekulargewicht | 70 kDa |
| Beobachtetes Molekulargewicht | 70 kDa |
| GenBank-Zugangsnummer | BC007276 |
| Gene symbol | HSC70 |
| Gene ID (NCBI) | 3312 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-G-Reinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
HSPA8 (also known as HSC70) is a member of the HSPA (HSP70) family of heat-shock proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA8 is a constitutively expressed cytosol/nuclear protein able to translocate between cytoplasm and nucleus. Recently it has been reported that HSPA8 can interact with α-synuclein, the critical pathological protein of Parkinson's disease, indicating its implication in neurodegenerative disease. (PMID: 21832061)
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for Hsc70 antibody 66442-1-Ig | Protokoll herunterladen |
| IHC protocol for Hsc70 antibody 66442-1-Ig | Protokoll herunterladenl |
| IF protocol for Hsc70 antibody 66442-1-Ig | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
J Neuroinflammation p38-TFEB pathways promote microglia activation through inhibiting CMA-mediated NLRP3 degradation in Parkinson's disease. | ||
Microbiol Spectr Heat Shock Protein Member 8 (HSPA8) Is Involved in Porcine Reproductive and Respiratory Syndrome Virus Attachment and Internalization. | ||
Cell Commun Signal Discovery of LAMP-2A as potential biomarkers for glioblastoma development by modulating apoptosis through N-CoR degradation. | ||
FEBS Open Bio A curcumin analogue GO-Y030 depletes cancer stem cells by inhibiting the interaction between the HSP70/HSP40 complex and its substrates | ||
Ecotoxicol Environ Saf PARP1 promotes NLRP3 activation via blocking TFEB-mediated autophagy in rotenone-induced neurodegeneration | ||
J Transl Med Arginine methylation of HSPA8 by PRMT9 inhibits ferroptosis to accelerate hepatitis B virus-associated hepatocellular carcinoma progression |







