HuR/ELAVL1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

HuR/ELAVL1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

CloneNo.

1A11H2

Kat-Nr. : 66549-1-Ig

Synonyme

ELAV like protein 1, ELAV1, ELAVL1, Hu antigen R, Hua, HuR, MelG

---

• 

  WB analysis using 66549-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66549-1-Ig (HuR/ELAVL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis of HEK-293 using 66549-1-Ig

  WB result of HuR/ELAVL1 antibody (66549-1-Ig; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HuR transfected HEK-293 cells.

• 

  WB analysis using 66549-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66549-1-Ig (HuR/ELAVL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66549-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66549-1-Ig (HuR antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 66549-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66549-1-Ig (HuR antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66549-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66549-1-Ig (HuR antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 66549-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66549-1-Ig (HuR antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66549-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HuR/ELAVL1 antibody (66549-1-Ig, Clone: 1A11H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HEK-293-Zellen, Jurkat-Zellen, MCF-7-Zellen, NCI-H1299-Zellen, Neuro-2a-Zellen, NIH/3T3-Zellen, PC-12-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 3 publications below

Produktinformation

66549-1-Ig bindet in WB, IHC, IF/ICC, ELISA HuR/ELAVL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HuR/ELAVL1 fusion protein Ag2249
• Vollständiger Name: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)
• Berechnetes Molekulargewicht: 326 aa, 36 kDa
• Beobachtetes Molekulargewicht: 34-37 kDa
• GenBank-Zugangsnummer: BC003376
• Gene symbol: HuR/ELAVL1
• Gene ID (NCBI): 1994
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ELAVL1, also named as HUR, belongs to the RRM elav family. It is involved in 3'-UTR ARE-mediated MYC stabilization. ELAVL1 binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, ELAVL1 binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.

Publikationen

Species	Application	Title
human	WB	Biochim Biophys Acta Mol Cell Res; IL-17 promotes melanoma through TRAF2 as a scaffold protein recruiting PIAS2 and ELAVL1 to induce EPHA5; Authors - Junfeng Du
mouse	WB,IF	Mol Cell Endocrinol; ELAV-like RNA binding protein 1 regulates osteogenesis in diabetic osteoporosis: Involvement of divalent metal transporter 1.; Authors - Yuanfei Ren
human	WB	Cell Signal; Butyrate mediated regulation of RNA binding proteins in the post-transcriptional regulation of inflammatory gene expression.; Authors - Aydan Torun
human	WB	J Int Med Res; Long non-coding RNA DEPDC1-AS1 promotes proliferation and migration of human gastric cancer cells HGC-27 via the human antigen R-F11R pathway.; Authors - Wei Xu
human	WB	Cell Death Dis; LncGMDS-AS1 promotes the tumorigenesis of colorectal cancer through HuR-STAT3/Wnt axis; Authors - Deji Ye ; KD Validated
human	IF	Oncol Lett; Stress granules affect the sensitivity of renal cancer cells to sorafenib by sequestering and stabilizing COX‑2 mRNA; Authors - Huiqi Dai