Human IgA Monoklonaler Antikörper

Human IgA Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3A5A5

Kat-Nr. : 60099-1-PBS

Synonyme

Human IgA Heavy Chain, IGHA1, 3A5A5, IgA, IgA1

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  WB analysis of human saliva using 60099-1-Ig (same clone as 60099-1-PBS)

  human saliva were subjected to SDS PAGE followed by western blot with 60099-1-Ig (Human IgA Antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60099-1-PBS in a different storage buffer formulation.

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  WB analysis of human plasma using 60099-1-Ig (same clone as 60099-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 60099-1-Ig (Human IgA Antibody) at dilution of 1:64000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60099-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60099-1-Ig (same clone as 60099-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60099-1-Ig (Human IgA antibody) at dilution of 1:320000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60099-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60099-1-Ig (same clone as 60099-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60099-1-Ig (Human IgA antibody) at dilution of 1:320000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60099-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60099-1-Ig (same clone as 60099-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60099-1-Ig (Human IgA antibody) at dilution of 1:50000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60099-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 60099-1-Ig (same clone as 60099-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Human IgA antibody (60099-1-Ig, Clone: 3A5A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60099-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60099-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA Human IgA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Human IgA fusion protein Ag7417
• Vollständiger Name: immunoglobulin heavy constant alpha 1
• Berechnetes Molekulargewicht: 496 aa, 53 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC016369
• Gene symbol: Human IgA Heavy Chain
• Gene ID (NCBI): 3493
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. This antibody recognizes the Chain C region of human Ig alpha-1 (IGHA1).